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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

Amaq Fadholly1, Arif Nur Muhammad Ansori1, Annise Proboningrat1, Alexander Patera Nugraha2, Regina Purnama Dewi Iskandar2, Fedik Abdul Rantam3, Sri Agus Sudjarwo4★★ Corresponding author

  1. 1Doctoral Student of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.
  2. 2Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga, INDONESIA.
  3. 3Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.
  4. 4Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.

CORRESPONDENCE

Sri Agus Sudjarwo

Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.

ags158@yahoo.com

Received: 25-09-2019; Revised: 26-12-2019; Accepted: 05-02-2020.

Volume 54, Issue 2 · pp. 416–421 · PUBLISHED 2020 · DOI: 10.5530/ijper.54.2.47

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ABSTRACT

Background: Annona squamosa is reported has a significant cytotoxic activity in some cancer cells. Objectives: Thus, this study aim to investigate Annona squamosa leaf extract induced by chitosan nanoparticles (nano-ASLE) to enhance their biological activity as anticancer agent on HeLa cells. Methods: Nano-ASLE (50, 100, 200, 400 μg/mL in DMSO) given on HeLa cells to determined IC50 value by MTT assay. Then, it was devided into three groups as follow IC50, 2IC50, 4IC50 continued with analysis of caspase-3 expression. Results: The present study demonstrated that nano-ASLE can surpress HeLa cells proliferation with the IC50 value of 344.48 μg/mL and rapid enhancement of caspase-3 activity has the mean score of 65.3 cell expression and the lowest score shows 45.3 cell expression. Conclusion: Nano-ASLE lead to HeLa cell death via the mitochondrial pathway on caspase-3 expression. In addition, the further studies are needed to obtain the loading efficiency, release of drug concentration and in vivo study of nano-ASLE to suppress HeLa cells.

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    Fadholly, A., Ansori, A. N. M., Proboningrat, A., Nugraha, A. P., Iskandar, R. P. D., Rantam, F. A., & Sudjarwo, S. A. (2020). Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study. Indian Journal of Pharmaceutical Education and Research, 54(2), 416–421. https://doi.org/10.5530/ijper.54.2.47