Article · Volume 54, Issue 2 · pp. 416–421
Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study
Amaq Fadholly1, Arif Nur Muhammad Ansori1, Annise Proboningrat1, Alexander Patera Nugraha2, Regina Purnama Dewi Iskandar2, Fedik Abdul Rantam3, Sri Agus Sudjarwo4
- 1Doctoral Student of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.
- 2Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga, INDONESIA.
- 3Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.
- 4Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, INDONESIA.
Abstract
Background: Annona squamosa is reported has a significant cytotoxic activity in some cancer cells.Objectives: Thus, this study aim to investigate Annona squamosa leaf extract induced by chitosan nanoparticles (nano-ASLE) to enhance their biological activity as anticancer agent on HeLa cells.Methods: Nano-ASLE (50, 100, 200, 400 μg/mL in DMSO) given on HeLa cells to determined IC50 value by MTT assay. Then, it was devided into three groups as follow IC50, 2IC50, 4IC50 continued with analysis of caspase-3 expression.Results: The present study demonstrated that nano-ASLE can surpress HeLa cells proliferation with the IC50 value of 344.48 μg/mL and rapid enhancement of caspase-3 activity has the mean score of 65.3 cell expression and the lowest score shows 45.3 cell expression.Conclusion: Nano-ASLE lead to HeLa cell death via the mitochondrial pathway on caspase-3 expression. In addition, the further studies are needed to obtain the loading efficiency, release of drug concentration and in vivo study of nano-ASLE to suppress HeLa cells.
Keywords: Annona squamosa; Cytotoxicity; Apoptosis; Caspase-3; Nanoparticles
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