Article · Volume 54, Issue 2 · pp. 440–447
LC-MS/MS Determination and Pharmacokinetics Study of Apremilast after Oral Administration in Rabbits
Tammisetty Mohan Rao1, Challa Balasekhara Reddy2, Puttagunta Srinivasa Babu3
- 1Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.
- 2Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy, Gurajala, Guntur, Andhra Pradesh, INDIA.
- 3Department of Pharmaceutics, Vignan Pharmacy College, Vadlamudi, Guntur, Andhra Pradesh, INDIA.
Abstract
Objectives: A selective and reproducible method has been optimised for evaluation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS.Methods: The analytical technique was implemented to quantify the apremilast in rabbit plasma samples with Apremilast-D5 as deuterated internal standard. The chromatographic separation tuned with 10mM Ammonium Acetate Buffer (pH: 4.0): Methanol: Acetonitrile, (20:40:40%, v/v/v) using the CORTECS C18, 2.7 μ.m, 4.6 m.m X 150 m.m analytical column with analysis time four minutes. The flow of mobile phase through column is 0.5 m.L/min. The mass spectrometric ions of Apremilast and Apremilast-D5 obtained were m/z 461.5→257.1 and 466.5→257.1.Results: The curve indicates correlation coefficient (r2) was superior than 0.998 with linear range of 0.03-48.0 n.g/m.L. The developed method was tuned to apply efficaciously for analyzing the pharmacokinetic parameters of Apremilast in rabbit plasma samples.Conclusion: An accurate and reproducible novel method was fabricated for estimation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS will be used for regular analysis and appropriate for therapeutic drug monitoring.
Keywords: Apremilast; Rabbit plasma; UPLC-ESI-MS/MS; Bio-analysis; Pharmacokinetic; Therapeutic drug monitoring
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