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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

LC-MS/MS Determination and Pharmacokinetics Study of Apremilast after Oral Administration in Rabbits

Tammisetty Mohan Rao1★, Challa Balasekhara Reddy2, Puttagunta Srinivasa Babu3★ Corresponding author

  1. 1Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.
  2. 2Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy, Gurajala, Guntur, Andhra Pradesh, INDIA.
  3. 3Department of Pharmaceutics, Vignan Pharmacy College, Vadlamudi, Guntur, Andhra Pradesh, INDIA.

CORRESPONDENCE

Tammisetty Mohan Rao

Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.

mohanjntuk.sch@gmail.com

Received: 16-07-2019; Revised: 24-09-2019; Accepted: 21-01-2020.

Volume 54, Issue 2 · pp. 440–447 · PUBLISHED 2020 · DOI: 10.5530/ijper.54.2.50

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ABSTRACT

Objectives: A selective and reproducible method has been optimised for evaluation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS. Methods: The analytical technique was implemented to quantify the apremilast in rabbit plasma samples with Apremilast-D5 as deuterated internal standard. The chromatographic separation tuned with 10mM Ammonium Acetate Buffer (pH: 4.0): Methanol: Acetonitrile, (20:40:40%, v/v/v) using the CORTECS C18, 2.7 μ.m, 4.6 m.m X 150 m.m analytical column with analysis time four minutes. The flow of mobile phase through column is 0.5 m.L/min. The mass spectrometric ions of Apremilast and Apremilast-D5 obtained were m/z 461.5→257.1 and 466.5→257.1. Results: The curve indicates correlation coefficient (r2) was superior than 0.998 with linear range of 0.03-48.0 n.g/m.L. The developed method was tuned to apply efficaciously for analyzing the pharmacokinetic parameters of Apremilast in rabbit plasma samples. Conclusion: An accurate and reproducible novel method was fabricated for estimation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS will be used for regular analysis and appropriate for therapeutic drug monitoring.

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    Rao, T. M., Reddy, C. B., & Babu, P. S. (2020). LC-MS/MS Determination and Pharmacokinetics Study of Apremilast after Oral Administration in Rabbits. Indian Journal of Pharmaceutical Education and Research, 54(2), 440–447. https://doi.org/10.5530/ijper.54.2.50