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IJPER · Lens · Curcumin Loaded Eudragit S100/PLGA Nanoparticles in Treatment of Colon Cancer: Formulation, Optimization, and in-vitro Cytotoxicity Study

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Article · Volume 55, Issue 2S · pp. s428–s440

Curcumin Loaded Eudragit S100/PLGA Nanoparticles in Treatment of Colon Cancer: Formulation, Optimization, and in-vitro Cytotoxicity Study

Apeksha Saraf1, Nidhi Dubey1, Nitin Dubey2, Mayank Sharma3

  1. 1School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road, Indore, Madhya Pradesh, INDIA.
  2. 2College of Pharmacy, IPS Academy, AB Road, Indore, Madhya Pradesh, INDIA.
  3. 3SVKM’s NMIMS School of Pharmacy and Technology Management, Shirpur, Dhule, Maharashtra, INDIA.

Abstract

Introduction: Polymeric nanoparticles provide a promising strategy for site-specific delivery of dietary phytochemicals in the treatment of colon cancer. Curcumin (CU) is a dietary phytochemical with well-proven anti-cancer activity in colon cancer. However, its clinical application is confined because of its hydrophobicity, lack of selectivity towards tumor tissues, and poor bioavailability.Objectives: The main objective of the study was to enhance the selectivity and cytotoxicity of poorly water-soluble curcumin by fabricating polymeric nanoparticles (NPs).Methods: In the present investigation, localized delivery of CU to the colon was achieved by employing a combination of Eudragit S100 (ES100), as a pH-sensitive polymer and polylactic-co-glycolic acid (PLGA), as a biodegradable polymer. The curcumin loaded dual-functional NPs were prepared by nanoprecipitation method and optimized using Box-Behnken experimental design.Results: The in-vitro cytotoxicity study of Curcumin Nanoparticles (CU-NPs) in CT26 murine colon carcinoma cells showed higher cytotoxicity in comparison to free drugs. The IC50 values of free curcumin and NPs containing curcumin was found to be 1.43±0.08 μg/ml and 0.25± 0.12 μg/ml, respectively (p <0.05).Conclusion: The results of the study suggested that the dual functional polymeric NPs exhibited a remarkably promising carrier system for effective delivery of such poorly water-soluble dietary phytochemicals in colon cancer.

Keywords: Colon cancer; Curcumin; Nanoparticles; Cytotoxicity; Nanoprecipitation; Box-Behnken

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