Curcumin Loaded Eudragit S100/PLGA Nanoparticles in Treatment of Colon Cancer: Formulation, Optimization, and in-vitro Cytotoxicity Study
Apeksha Saraf1★, Nidhi Dubey1, Nitin Dubey2, Mayank Sharma3★ Corresponding author
- 1School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road, Indore, Madhya Pradesh, INDIA.
- 2College of Pharmacy, IPS Academy, AB Road, Indore, Madhya Pradesh, INDIA.
- 3SVKM’s NMIMS School of Pharmacy and Technology Management, Shirpur, Dhule, Maharashtra, INDIA.
CORRESPONDENCE
Apeksha Saraf
School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road, Indore, Madhya Pradesh, INDIA.
Received: 14-09-2020; Revised: 20-01-2021; Accepted: 24-04-2021.
Volume 55, Issue 2S · pp. s428–s440 · PUBLISHED 2021 · DOI: 10.5530/ijper.55.2s.114
ABSTRACT
Introduction: Polymeric nanoparticles provide a promising strategy for site-specific delivery of dietary phytochemicals in the treatment of colon cancer. Curcumin (CU) is a dietary phytochemical with well-proven anti-cancer activity in colon cancer. However, its clinical application is confined because of its hydrophobicity, lack of selectivity towards tumor tissues, and poor bioavailability. Objectives: The main objective of the study was to enhance the selectivity and cytotoxicity of poorly water-soluble curcumin by fabricating polymeric nanoparticles (NPs). Methods: In the present investigation, localized delivery of CU to the colon was achieved by employing a combination of Eudragit S100 (ES100), as a pH-sensitive polymer and polylactic-co-glycolic acid (PLGA), as a biodegradable polymer. The curcumin loaded dual-functional NPs were prepared by nanoprecipitation method and optimized using Box-Behnken experimental design. Results: The in-vitro cytotoxicity study of Curcumin Nanoparticles (CU-NPs) in CT26 murine colon carcinoma cells showed higher cytotoxicity in comparison to free drugs. The IC50 values of free curcumin and NPs containing curcumin was found to be 1.43±0.08 μg/ml and 0.25± 0.12 μg/ml, respectively (p <0.05). Conclusion: The results of the study suggested that the dual functional polymeric NPs exhibited a remarkably promising carrier system for effective delivery of such poorly water-soluble dietary phytochemicals in colon cancer.
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Saraf, A., Dubey, N., Dubey, N., & Sharma, M. (2021). Curcumin Loaded Eudragit S100/PLGA Nanoparticles in Treatment of Colon Cancer: Formulation, Optimization, and in-vitro Cytotoxicity Study. Indian Journal of Pharmaceutical Education and Research, 55(2S), s428–s440. https://doi.org/10.5530/ijper.55.2s.114