Micellar Enhanced Spectrofluorimetric Quantification of Gemifloxacin Mesylate in Pharmaceuticals and Bio-fluids
Salma Ali Al-Tamimi1★, Amal Mohammed Al-Mohaimeed1, Nawal Ahmad Alarfaj1, Fatma Ahmed Aly2★ Corresponding author
- 1Department of Chemistry, College of Science, King Saud University, Riyadh, SAUDI ARABIA.
- 2Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, EGYPT.
CORRESPONDENCE
Salma Ali Al-Tamimi
Department of Chemistry, College of Science, King Saud University, Riyadh, SAUDI ARABIA.
Received: 06-05-2021; Revised: 23-08-2021; Accepted: 29-12-2021.
Volume 56, Issue 1S · pp. 1–8 · PUBLISHED · DOI: 10.5530/ijper.56.1s.37
ABSTRACT
Introduction: Gemifloxacin mesylate (GFX) is one of the most potential anti-infective agents with broad spectrum and influential activity. Objectives: The current study described a fast and precise spectrofluorometric probe for GFX quantification in authentic drug, commercial tablets and bio-samples. Materials and Methods: The suggested technique was conducted by complexing GFX with Al ions in the presence of alkaline buffer of pH 8. Results: The FI was enhanced by adding sodium dodecyl sulfate (SDS). Rectilinear relationship was achieved over (0.05-100 ng mL-1) drug samples. The fluorescence spectra were recorded at λex at 268 nm and λem 400 nm. The lowest quantification and detection values were 0.049 ng mL-1 and 0.05-100 ng mL-1, respectively. The suggested probe was employed to determine the investigated drug in its Factive® tablets with percentage recovery 99.85±0.7 %. In addition, the selected drug was also quantified in human serum and urine with percentage recovery 99.4 %. The outcome data were statistically treated and validated. Conclusion: The obtained results confirmed the suitability of this probe for the quantification of the drug in bio-fluids and commercial tablets.
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Al-Tamimi, S. A., Al-Mohaimeed, A. M., Alarfaj, N. A., & Aly, F. A. (). Micellar Enhanced Spectrofluorimetric Quantification of Gemifloxacin Mesylate in Pharmaceuticals and Bio-fluids. Indian Journal of Pharmaceutical Education and Research, 56(1S), 1–8. https://doi.org/10.5530/ijper.56.1s.37