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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

LC-ESI-MS/MS Method for the Fosamprenavir Quantification Bioanalytical Method Development and Validation for the Quantification Fosamprenavir in Human Plasma by LC-ESI-MS/MS

Kondam Susmitha1★, Muthukkarupan Menaka2★ Corresponding author

  1. 1Department of Pharmaceutical Chemistry, Annamalai University, Chidambaram, Tamil Nadu, INDIA.
  2. 2Department of Pharmaceutics, Annamalai University, Chidambaram, Tamil Nadu, INDIA.

CORRESPONDENCE

Kondam Susmitha

Department of Pharmaceutical Chemistry, Annamalai University, Chidambaram, Tamil Nadu, INDIA.

kondamsusmithadoer@gmail.com

Received: 06-11-2020; Revised: 12-12-2021; Accepted: 04-06-2022.

Volume 56, Issue 3S · pp. S599–S604 · PUBLISHED 2022 · DOI: 10.5530/ijper.56.3s.168

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ABSTRACT

Objectives: The main goal of the current research was to develop a bioanalytical technique for the quantification of fosamprenavir in plasma by LC-ESI-MS/MS. Materials and Methods: Chromatographic elution was attained thru a Zorbax (3.5 μm; 50×4.6 mm) analytical C18-column with isocratic system by methanol, 0.1%v/v formic acid and acetonitrile in the ratio of 60:10:30 V/V as mobile phase with flowrate of 0.70 ml/ min. Liquid-liquid extraction was executed for the drug separation with an ethyl acetate solvent. Parent and product ions were monitored at m/z 586.19 /57.0 for Fosamprenavir and 590.0/61.0 for Fosamprenavir-D4 on MRM. Results: Linearity plot of fosamprenavir was rectilinear over the concentration of 4.0- 1600.0 ng/ml with correlation coefficient (r2) value of more than 0.99. The developed procedure has fine recovery with percentage recovery findings of HQC, MQC and LQC standards were present between 89.65% to 95.61%. The % RSD findings were < 6.30% for intra-day and inter-day accuracy and precision. Conclusion: Fosamprenavir has more stability for longer time when subjected for different stability environments and the technique was effectively relevant to routine analysis of fosamprenavir in biological matrix.

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    Susmitha, K., & Menaka, M. (2022). LC-ESI-MS/MS Method for the Fosamprenavir Quantification Bioanalytical Method Development and Validation for the Quantification Fosamprenavir in Human Plasma by LC-ESI-MS/MS. Indian Journal of Pharmaceutical Education and Research, 56(3S), S599–S604. https://doi.org/10.5530/ijper.56.3s.168