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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

AmruthMune, A Standardized Extract Comprises of Tinosporin from Tinospora cordifolia and its Mechanism of Immunostimulatory Activity

Kuluvar Gouthamchandra1★, Sudeep Heggar Venkataramana1, Amritharaj Amritharaj1, Anusha Sathish1, Lingaraju Harakanahalli Basavegowda2, Naveen Puttaswamy3, Shyam Prasad Kodimule2,3★ Corresponding author

  1. 1Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd., Bangalore, Karnataka, INDIA.
  2. 2Department of Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd., Bangalore, Karnataka, INDIA.
  3. 3Department of Analytical Development Research Lab, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd., Bangalore, Karnataka, INDIA.

CORRESPONDENCE

Kuluvar Gouthamchandra

Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd., Bangalore, Karnataka, INDIA.

goutham06@gmail.com

Received: 28-02-2022; Revised: 12-07-2022; Accepted: 03-10-2022.

Volume 57, Issue 2 · pp. 490–495 · PUBLISHED 2023 · DOI: 10.5530/ijper.57.2.59

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ABSTRACT

Background: Tinospora cordifolia is an illustrious medicinal herb in Indian traditional ayurvedic medicine because of its substantial medicinal properties. It is reported that Tinospora cordifolia has been used to cure for many ailments by traditional medicinal practitioners. It is captivating that, in this contemporary world Tinospora cordifolia is being used to retain good immune health. Materials and Methods: The objective of this research is to demonstrate the immune enhancing activity of AmruthMune a standardized extract of T. cordifolia extract with Tinosporin. MTT assay, NO assay, H2 O2 assay, Phagocytosis assay, ELISA, and western blotting experiments were conducted in this study to understand the efficacy and its basic molecular mechanism. Results: Quantitative HPLC analysis determined that the AmruthMune a standardized Tinospora cordifolia extract comprise of active principle Tinosporin (1%). In addition, AmruthMune treatment dramatically improved the phagocytosis and secretion of H2 O2 NO, TNF-α, and IL-6 in macrophage cells. Furthermore, consequences of western blot experiments demonstrated that AmruthMune treatment activates p-ERK, p-p38 and p-JNK. Conclusion: Collectively, these results exhibited MAPK signalling pathway involved in AmruthMune elicited macrophage activation and it could be a promising functional food in immune modulation.

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    Gouthamchandra, K., Venkataramana, S. H., Amritharaj, A., Sathish, A., Basavegowda, L. H., Puttaswamy, N., & Kodimule, S. P. (2023). AmruthMune, A Standardized Extract Comprises of Tinosporin from Tinospora cordifolia and its Mechanism of Immunostimulatory Activity. Indian Journal of Pharmaceutical Education and Research, 57(2), 490–495. https://doi.org/10.5530/ijper.57.2.59