Synthesis and Evaluation of 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells

Abstract

Aim To carry out Synthesis and Evaluation of novel 2, 5-substituted Pyrazolonefor Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells. Materials and Methods Alzheimer’s Disease (AD) has become a serious public health concern as a result of people living longer than ever before. It is critically necessary to discover a way to halt and postpone the illness. The present study aims to determine if new synthetic analogue pyrazole derivatives may protect against toxicity caused by Aβ25-35 and its underlying mechanisms in neuroblastoma cells like SH-SY5Y. PyRx 0.9 software was used for molecular docking studies. The cells of SH-SY5Y were preincubated for 30 min with varying doses of the generated compounds (C1-C10) to induce neurotoxicity. They were then grown in Aβ25-35 (25 mol/L) for 48 hr. Cell viability was determined by MTT assay. Results Compounds C5 and C8 exhibited a better binding score -8.4k/cal compared to other analogues. Synthesized compounds C5 and C8 inhibited Aβ25-35-induced apoptosis in SH-SY5Y cells and protected neural cells from damage. Conclusion The MTT assay confirmed that compounds C5 and C8 significantly reduced Aβ25-35-induced toxicity among human neuroblastoma SH-SY5Y cells, demonstrating its neuroprotective properties.

Keywords: Cell viability; MTT assay; Neurodegenerative diseases; Neuroprotective agent; Pyrazole derivatives; SH-SY5Y cells