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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Synthesis and Evaluation of 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells

R. Manikandan1★, K. Devi1, D. Rajalingam2★ Corresponding author

  1. 1Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, INDIA.
  2. 2Department of Pharmaceutical Chemistry, Kamalakshi Pandurangan College of Pharmacy, Tiruvannamalai, Tamil Nadu, INDIA.

CORRESPONDENCE

R. Manikandan

Research Scholar, Department of Pharmacy, Faculty of Engineering & Technology, Annamalai University, Annamalai Nagar-608002, Chidambaram, Tamil Nadu, INDIA.

rmanipharmacy@gmail.com

Received: 28-11-2023; Revised: 31-01-2024; Accepted: 01-02-2024.

Volume 59, Issue 1S · pp. s323–s332 · PUBLISHED Jan-Mar 2025 · DOI: 10.5530/ijper.20256349

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ABSTRACT

Aim To carry out Synthesis and Evaluation of novel 2, 5-substituted Pyrazolonefor Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells. Materials and Methods Alzheimer’s Disease (AD) has become a serious public health concern as a result of people living longer than ever before. It is critically necessary to discover a way to halt and postpone the illness. The present study aims to determine if new synthetic analogue pyrazole derivatives may protect against toxicity caused by Aβ25-35 and its underlying mechanisms in neuroblastoma cells like SH-SY5Y. PyRx 0.9 software was used for molecular docking studies. The cells of SH-SY5Y were preincubated for 30 min with varying doses of the generated compounds (C1-C10) to induce neurotoxicity. They were then grown in Aβ25-35 (25 mol/L) for 48 hr. Cell viability was determined by MTT assay. Results Compounds C5 and C8 exhibited a better binding score -8.4k/cal compared to other analogues. Synthesized compounds C5 and C8 inhibited Aβ25-35-induced apoptosis in SH-SY5Y cells and protected neural cells from damage. Conclusion The MTT assay confirmed that compounds C5 and C8 significantly reduced Aβ25-35-induced toxicity among human neuroblastoma SH-SY5Y cells, demonstrating its neuroprotective properties.

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    Manikandan, R., Devi, K., & Rajalingam, D. (2025). Synthesis and Evaluation of 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells. Indian Journal of Pharmaceutical Education and Research, 59(1S), s323–s332. https://doi.org/10.5530/ijper.20256349