jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
International Journal of Pharmaceutical InvestigationNOW IN
Article

Cloning and Expression of Helicobacter pylori ulcer Associated Gene - iceA1 in Brinjal (Solanum melongena L.)

Mohammad Javad Mehran1, Basaralu Yadurappa Sathish Kumar1,2★, Nanjundappa Haraprasad3, Rambod Barzigar1, Bashasab Fakrudin4, Sayan Paul5,6★ Corresponding author

  1. 1JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.
  2. 2Department of Biotechnology, JSS College, Ooty Road, Mysore, Karnataka, INDIA.
  3. 3JSS Science and Technology University, SJCE, Technical Campus, Mysore, Karnataka, INDIA.
  4. 4Department of Biotechnology and Crop Improvement, College of Horticulture, University of Horticulture Sciences Campus, GKVK Post, Bengaluru, Karnataka, INDIA.
  5. 5Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, INDIA.
  6. 6Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, INDIA.

CORRESPONDENCE

Basaralu Yadurappa Sathish Kumar

JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.

bysathish@gmail.com

Received: 25-08-2021; Revised: 10-09-2021; Accepted: 10-09-2021.

Volume 11, Issue 3 · pp. 338–344 · PUBLISHED · DOI: 10.5530/ijpi.2021.3.60

View on IJPI original site ↗

ABSTRACT

Background:Plant biotechnology revolutionized the branch of plant sciences contributing to improving crop production. Helicobacter pylori was predominantly seen among all the sectors of the human population, closely stuck to stomach ulcers and cancer. Even though traditional vaccines are effective in preventing H. pylori, edible and non-edible parts of special transgenic plants were also being generated to express its antigens which could elicit an immune response after consuming. The induce by contact with epithelium (iceA1) protein of H. pylori is associated with virulence and stated as predisposing factor to stomach cancer. Objectives: This study intends to study the expression levels of iceA1 gene in the brinjal plant. Materials and Methods: The present study, magnified the iceA1 gene from H. pylori strain 26695 and transformed it into callus of brinjal (from leaf explants) through Agrobacterium tumefacians (EHA105). pBI121 vector was used to construct the plant expression vector, and the transgenic plants generated were further validated through gene amplification and protein expression studies. Results: Out of the 46 plants obtained from this study, five of them were found to be positive for the iceA1 expression as seen on validation assays like gene amplification and protein expression studies. Conclusion:The study could beneficially produce a vaccine candidate against H. pylori from transgenic brinjal. In Addition, it could provide, valid scientific data which can further be used by researchers.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Mehran, M. J., Kumar, B. Y. S., Haraprasad, N., Barzigar, R., Fakrudin, B., & Paul, S. (). Cloning and Expression of Helicobacter pylori ulcer Associated Gene - iceA1 in Brinjal (Solanum melongena L.). International Journal of Pharmaceutical Investigation, 11(3), 338–344. https://doi.org/10.5530/ijpi.2021.3.60