Article · Volume 1, Issue 4 · pp. 371–374

Determination of Progesterone in Capsules by High-Performance Liquid Chromatography and UV- Spectrophotometry

Maliwal D¹, Jain P¹, Jain A¹, Patidar V²

¹Department of Pharmaceutical Analysis, B.R. Nahata Contract Research Centre, Mandsaur, INDIA.
²Department of Pharmaceutics, College of Pharmacy, IPS Academy, Indore, INDIA.

Abstract

The rapid, simple, and accurate chromatographic (high-performance liquid chromatography) and spectrophotometric method for the determination of progesterone in capsule was elaborated. Methanol was found to be a suitable extraction solvent. The samples were chromatographed on Linchrocart C18 column and UV detection at 254 nm. The elution was achieved isocratically with a mobile phase of methanol - water (80:20, v/v). The method was validated for precision, linearity, accuracy, and limit of detection.

Keywords: Analysis in capsules; progesterone; reversed-phase chromatography; UV-spectrophotometry

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