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Article

Determination of Progesterone in Capsules by High-Performance Liquid Chromatography and UV- Spectrophotometry

Maliwal D1★, Jain P1, Jain A1, Patidar V2★ Corresponding author

  1. 1Department of Pharmaceutical Analysis, B.R. Nahata Contract Research Centre, Mandsaur, INDIA.
  2. 2Department of Pharmaceutics, College of Pharmacy, IPS Academy, Indore, INDIA.

CORRESPONDENCE

Maliwal D

Department of Pharmaceutical Analysis, B.R. Nahata Contract Research Centre, Mandsaur, INDIA.

diyampharm@gmail.com

Volume 1, Issue 4 · pp. 371–374 · PUBLISHED · DOI: 10.4103/0975-1483.59330

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ABSTRACT

The rapid, simple, and accurate chromatographic (high-performance liquid chromatography) and spectrophotometric method for the determination of progesterone in capsule was elaborated. Methanol was found to be a suitable extraction solvent. The samples were chromatographed on Linchrocart C18 column and UV detection at 254 nm. The elution was achieved isocratically with a mobile phase of methanol - water (80:20, v/v). The method was validated for precision, linearity, accuracy, and limit of detection.

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    D, M., P, J., A, J., & V, P. (). Determination of Progesterone in Capsules by High-Performance Liquid Chromatography and UV- Spectrophotometry. Journal of Young Pharmacists, 1(4), 371–374. https://doi.org/10.4103/0975-1483.59330