Polyclonal T Lymphocytes Transform into Malignant Cells by Retroviral Mediated Transfer of p21SNFT Gene
Ashok Kumar1★, Bharathi Sengodan1, Chetan Lal2, Anthony Leela3, Kevin Fernandez3★ Corresponding author
- 1Department of Pathology, Faculty of Medicine, AIMST University, MALAYSIA.
- 2Department of Microbiology, Federal Postgraduate Medical Institute, Lahore, Punjab, PAKISTAN.
- 3Department of Community Medicine, Faculty of Medicine, AIMST University, MALAYSIA.
CORRESPONDENCE
Ashok Kumar
Department of Pathology, Faculty of Medicine, AIMST University, MALAYSIA.
Received: 03-02-2020; Revised: 15-02-2020; Accepted: 17-03-2020.
Volume 12, Issue 1 · pp. 71–74 · PUBLISHED · DOI: 10.5530/jyp.2020.12.14
ABSTRACT
Background:p21SNFT (21 kDa small nuclear factor isolated from T cells) gene is known to be increasingly expressed in the cells of Hodgkin disease and Hodgkin cell lines. It was not investigated before if this gene had any effect on polyclonal T lymphocytes. For this purpose, we transduced this gene into polyclonal T lymphocytes and transplanted into mice. Materials and Methods:We used γ-retroviral vector to transduce this gene into polyclonal T lymphocytes, isolated from wild type black-6 mice. After confirmation of p21SNFT expression via marker gene eGFP (enhanced green fluorescent protein) and Western blot analysis, these cells were transplaned into immunocompromised Rag 1 deficient mice. The control group of mice was transplanted with γ-retroviral vector, carrying only marker gene (eGFP). The mice were followed up. The results showed that all 7 mice transplanted with p21SNFT-carrying polyclonal T lymphocytes developed massively enlarged mesenteric lymph nodes after 435 to 550 days of transplantation.Results: Histopathological analyses of these lymph nodes revealed the lymphoma, which resembled human peripheral T cell lymphoma type. Furthermore, immunophenotyping of tumor cells by FACS analysis revealed loss of T cell markers and expression of CD19 marker. From the control group, all five mice remained healthy and did not develop any tumor. Conclusion:The study concludes that γ-retroviral mediated transfer of p21SNFT transforms polyclonal T lymphocytes into malignant cells.
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Kumar, A., Sengodan, B., Lal, C., Leela, A., & Fernandez, K. (). Polyclonal T Lymphocytes Transform into Malignant Cells by Retroviral Mediated Transfer of p21SNFT Gene. Journal of Young Pharmacists, 12(1), 71–74. https://doi.org/10.5530/jyp.2020.12.14