Article · Volume 2, Issue 4 · pp. 414–416

Visible Spectrophotometric Estimation of Diacerein in Bulk and Pharmaceutical Dosage Forms

Sivakumar R¹, Nallasivan PK¹, Saranya KC¹, Sam Solomon WD¹, Akelesh T¹, Venkatnarayanan R¹

¹Department of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamil Nadu, INDIA.

Abstract

Two simple, sensitive, accurate, rapid, and economical spectrophotometric methods have been developed for the estimation of diacerein in Pharmaceutical dosage forms. Method A is based on the reaction of diacerein with Folin-Ciocalteu reagent, in the presence of 0.5 N sodium hydroxide solution, giving a pink-colored chromogen, which shows maximum absorbance at 512 nm against reagent blank, while method B is based on the oxidation of diacerein with potassium permanganate in an alkaline medium giving a pink-colored chromogen, which shows maximum absorption at 497.5 nm. Beer's law was obeyed in the concentration range of 4 – 20 μg/ml for both methods A and B. Results of the analysis were validated statistically, and by recovery studies.

Keywords: Diacerein; FC reagent; KMnO4; spectrophotometry

The full text of this article is available as a PDF. Open the PDF.