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HPLC analysis and standardization of Brahmi vati - An Ayurvedic poly-herbal formulation

Amrita Mishra1,2★, Arun K. Mishra1, Om Prakash Tiwari1, Shivesh Jha2★ Corresponding author

  1. 1Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, INDIA.
  2. 2Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, INDIA.

CORRESPONDENCE

Amrita Mishra

Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, INDIA.

amrita_azam@rediffmail.com

Received: 06-06-2013; Revised: 05-09-2013; Accepted: 30-09-2013.

Volume 5, Issue 3 · pp. 77–82 · PUBLISHED · DOI: 10.1016/j.jyp.2013.09.001

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ABSTRACT

Objectives:The aim of the present study was to standardize Brahmi vati (BV) by simultaneous quantitative estimation of Bacoside A3 and Piperine adopting HPLCeUV method. BV very important Ayurvedic polyherbo formulation used to treat epilepsy and mental disorders containing thirty eight ingredients including Bacopa monnieri L. and Piper longum L. Materials and methods:An HPLCeUV method was developed for the standardization of BV in light of simultaneous quantitative estimation of Bacoside A3 and Piperine, the major constituents of B. monnieri L. and P. longum L. respectively. The developed method was validated on parameters including linearity, precision, accuracy and robustness. Results:The HPLC analysis showed significant increase in amount of Bacoside A3 and Piperine in the inhouse sample of BV when compared with all three different marketed samples of the same. Results showed variations in the amount of Bacoside A3 and Piperine in different samples which indicate nonuniformity in their quality which will lead to difference in their therapeutic effects. Conclusion:The outcome of the present investigation underlines the importance of standardization of Ayurvedic formulations. The developed method may be further used to standardize other samples of BV or other formulations containing Bacoside A3 and Piperine.

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    Mishra, A., Mishra, A. K., Tiwari, O. P., & Jha, S. (). HPLC analysis and standardization of Brahmi vati - An Ayurvedic poly-herbal formulation. Journal of Young Pharmacists, 5(3), 77–82. https://doi.org/10.1016/j.jyp.2013.09.001