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Journal of Young PharmacistsNOW IN
Communication

Diversity of endophytic bacteria in Malaysian plants as revealed by 16S rRNA encoding gene sequence based method of bacterial identification

Chye Ying Loh1, Yin Yin Tan1, Rahim Rohani2, Jean-Frédéric F. Weber2, Subhash Janardhan Bhore1★★ Corresponding author

  1. 1Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, MALAYSIA.
  2. 2Atta-ur-Rahman Research Institute of Natural Products for Drug Discovery (RiND) and Faculty of Pharmacy, MARA University of Technology (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, MALAYSIA.

CORRESPONDENCE

Subhash Janardhan Bhore

Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, MALAYSIA.

subhashbhore@gmail.com

Received: 18-05-2013; Revised: 02-07-2013; Accepted: 30-07-2013.

Volume 5, Issue 3 · pp. 95–97 · PUBLISHED · DOI: 10.1016/j.jyp.2013.07.001

View on JYP original site ↗

ABSTRACT

Bacterial endophytes do have several potential applications in pharmacy, medicine and agricultural biotech industry. The main objective of this study was to understand types of bacterial endophytes associated with dicotyledonous (dicot) and monocotyledonous (monocot) plant species. Isolation of the endophytic bacteria was performed using surface-sterilized various tissue samples, and identification of the endophytic bacterial isolates (EBIs) was completed using 16S rRNA encoding gene sequence similarity based method. In total, 996 EBIs were isolated and identified from 1055 samples of 31 monocot and 65 dicot plant species from Peninsular Malaysia. The 996 EBIs represented 71 different types of bacterial species. Twelve (12) out of 71 species are reported as endophytes for the first time. We conclude that diverse types of bacterial endophytes are associated with dicot and monocot plants, and could be useful in pharmacy, medicine and agricultural biotechnology for various potential applications.

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    Loh, C. Y., Tan, Y. Y., Rohani, R., Weber, J. F., & Bhore, S. J. (). Diversity of endophytic bacteria in Malaysian plants as revealed by 16S rRNA encoding gene sequence based method of bacterial identification. Journal of Young Pharmacists, 5(3), 95–97. https://doi.org/10.1016/j.jyp.2013.07.001