jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Journal of Young PharmacistsNOW IN
Communication

Hepatotoxicity by Acetaminophen and Amiodarone in Zebrafish Embryos

Mehul Pandya1★, Dipak Patel1, Jigar Rana1, Manish Patel1, Nadeem Khan1★ Corresponding author

  1. 1Department of Toxicology, Jai Research Foundation, Opp. National highway number 8, next to Daman-Ganga Bridge, Valvada, Di-Valsad, Gujrat, INDIA.

CORRESPONDENCE

Mehul Pandya

Department of Toxicology, Jai Research Foundation, Opp. National highway number 8, next to Daman-Ganga Bridge, Valvada, Di-Valsad, Gujrat, INDIA.

mehul.pandya@jrfonline.com

Volume 8, Issue 1 · pp. 50–52 · PUBLISHED · DOI: 10.5530/jyp.2016.1.11

View on JYP original site ↗

ABSTRACT

Introduction:Liver is an essential organ for the detoxification of chemicals and play pivotal role in various biochemical and physiological processes hence liver toxicity is one of the major causes for the withdrawal of newly discovered molecules. Characteristics viz. high fecundity rate, transparent eggs, less maintenance cost, livelihood in E3 medium without external feeding make Zebrafish (Danio rerio) suitable vertebral model for the hepatotoxicity assay. Methods: On 3 dpf, embryos were exposed to various concentrations of Acetaminophen and Amiodarone in well plates. This procedure was followed up to day 5. Due to mortality observed in Amiodarone treated group G32 (15μM) treatment was not performed for. On 6 dpf, embryos were kept in lateral position in agar plates and photography was performed. Captured images were analysed through Image J software. Results:Mortalities were observed in Amiodarone treated groups on day 4 (7.1 % at 25 μM), on day 5 (78.5% and 68.2% at 25 μM and 15 μM ) and day 6 (100%, 78.5% and 64.2% at 25 μM, 15 μM and 10 μM). Statistically significant decreased pixel intensity was observed at 1000 μM, 2000 μM and 5000 μM Acetaminophen concentrations and at 5 μM, 7 μM, 10 μM Amiodarone concentrations.Conclusion: Acetaminophen and Amiodarone have hepatotoxic potential in zebrafish embryos. Amiodarone induces embryo lethality at 10 μM. NOAEL for Amiodarone is 3 μM and for Acetaminophen is 500 μM.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Pandya, M., Patel, D., Rana, J., Patel, M., & Khan, N. (). Hepatotoxicity by Acetaminophen and Amiodarone in Zebrafish Embryos. Journal of Young Pharmacists, 8(1), 50–52. https://doi.org/10.5530/jyp.2016.1.11