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Article

Analysis of the metabolites of mesaconitine in rat blood using ultrafast liquid chromatography and electrospray ionization mass spectrometry

Peng Tan1, Xin Chen2, Ruirui He3, Yonggang Liu1★★ Corresponding author

  1. 1Beijing University of Chinese Medicine, China.
  2. 2Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, China.
  3. 3Institute of Clinical Pharmacology, Peking University, Beijing, China.

CORRESPONDENCE

Yonggang Liu

Beijing University of Chinese Medicine, China.

liuyg0228@163.com

Received: 15-02-2013; Revised: 10-01-2013.

Volume 10, Issue 38 · pp. 101–105 · PUBLISHED 17 April 2014 · DOI: 10.4103/0973-1296.131019

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ABSTRACT

Background: Mesaconitine is the main active component of genus aconitum plants that are widely used in clinics in China. However, little has been known about the metabolic pathway of mesaconitine. Objective: To explore the metabolites and propose the pathway of mesaconitine. Materials and Methods: In the present study, mesaconitine (4 mg kg−1) was orally administered to male rats. Then, blood samples collected were pretreated using solid-phase extraction technique with C18 cartridges, and analyzed using LC/MS/MS method with electrospray ionization. Both positive ion mode and collision induced dissociation (CID) were used to elucidate the structures of the major metabolites of mesaconitine. Results: Ten compounds were identifi ed, among which seven were new metabolites, and the metabolic pathway was proposed. The protonated molecular ions of seven new metabolites were at m/z 648, 618, 616, 602, 572, 468, and 542, multistage fragment ions with neutral loss of 28 u (CO), 60 u (CH3COOH), 18 u (H2O), and 32 u (CH3OH). These new metabolites detected fi rstly in vivo, were named 10-hydroxyl-mesaconitine, hypaconitine, dehydrated mesaconitine 16-O-demethylmesaconitine, 16-O-demethylhypaconitine, and 16-O-demethyl-dehydrated hypaconitine, respectively. Furthermore, the breaking sequence of methoxyl was obtained using quantum chemistry. Conclusion: The study proved that the method of solid-phase extraction technique coupled with MS and quantum chemistry can be applied to the analysis of metabolites in plasma quickly and conveniently.

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    Tan, P., Chen, X., He, R., & Liu, Y. (2014). Analysis of the metabolites of mesaconitine in rat blood using ultrafast liquid chromatography and electrospray ionization mass spectrometry. Pharmacognosy Magazine, 10(38), 101–105. https://doi.org/10.4103/0973-1296.131019