Simultaneous determination of four neuroprotective compounds of Tilia amurensis by high performance liquid chromatography coupled with diode array detector
Bohyung Lee2, Jin Bae Weon2, Bo‑Ra Yun2, Jiwoo Lee2, Min Rye Eom2, Choong Je Ma2,3★★ Corresponding author
- 1Department of Medical Biomaterials Engineering, College of Biomedical Science.
- 2Research Institute of Biotechnology, Kangwon National University, Chuncheon 200‑701, Republic of Korea.
CORRESPONDENCE
Choong Je Ma
Research Institute of Biotechnology, Kangwon National University, Chuncheon 200‑701, Republic of Korea.
Received: 16-09-2013; Revised: 14-01-2014.
Volume 10, Issue 39 · pp. 195–199 · PUBLISHED 24 July 2014 · DOI: 10.4103/0973-1296.137353
ABSTRACT
Background: Tilia amurensis consists of various compounds, such as flavonoids and terpenoids. Objective: A simple and reliable high performance liquid chromatography (HPLC) coupled with the diode array detector (DAD) method has been established for simultaneous determination of epicatechin, nudiposide, lyoniside, and scopoletin isolated from Tilia amurensis. Materials and Methods: Optimum separations were obtained with a SHISEIDO C18 column by gradient elution, with 0.1% Trifluoroacetic acid (TFA) water‑methanol as the mobile phase. The gradient elution system was completed within 40 minutes. The flow rate and detection wavelength were 1 mL/minute, 205 nm, 250 nm, and 280 nm, respectively. Results: Validation of the analytical method was evaluated by linearity, precision, and the accuracy test. The calibration curve was linear over the established range with R2 > 0.997. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01-15.20 μg/mL and 0.03-46.06 μg/mL. The method exhibited an intraday and interday precision range of 96.25-105.66% and 93.52-109.92%, respectively (RSD <2.80%). The recoveries and relative standard deviation (RSD) of the four compounds in Tilia amurensis were in the range of 90.42-104.84% and 0.2-2.58%. Conclusion: This developed method was accurate and reliable for the quality evaluation of the four compounds isolated from Tilia amurensis.
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Lee, B., Weon, J. B., Yun, B., Lee, J., Eom, M. R., & Ma, C. J. (2014). Simultaneous determination of four neuroprotective compounds of Tilia amurensis by high performance liquid chromatography coupled with diode array detector. Pharmacognosy Magazine, 10(39), 195–199. https://doi.org/10.4103/0973-1296.137353