A rapid UPLC method for simultaneous determination of eleven components in ‘Ge‑Gen‑Qin‑Lian’ decoction
Rui An1★, Lisha You1, Yizhu Zhang1, Xinhong Wang1, Yuemin Ma1★ Corresponding author
- 1Department of Chemistry, College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of, China.
CORRESPONDENCE
Rui An
Department of Chemistry, College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of, China.
Received: 15-08-2013; Revised: 02-09-2013.
Volume 10, Issue 40 · pp. 464–469 · PUBLISHED 26 September 2014 · DOI: 10.4103/0973-1296.141821
ABSTRACT
Background: ‘Ge‑Gen‑Qin‑Lian’ Decoction derived from ‘Shang‑Han‑Lun’ compiled by Zhang Zhongjing. It is widely used in the treatment of acute gastroenteritis, bacillary dysentery, virus diarrhea. This paper describes a sensitive and specific assay for the determination of the 11‑marker compounds using ultra performance liquid chromatography (UPLC). Objective: To develop an UPLC method for simultaneous determination of 11 bioactive compounds in ‘Ge‑Gen‑Qin‑Lian’ preparations. Materials and Methods: The chromatography analysis was performed on an Agilent Proshell 120 EC‑C18 column (4.6 × 50 mm, 2.7 μm) at 30°C with a gradient elution of methanol, 0.5% formic acid and 0.5% ammonium acetate at a flow rate 1.0 ml/min and UV detected at 270 nm. Results: All calibration curves showed good linear regression (r ≥ 0.9993) within tested ranges. Limits of detection (LOD) and limits of quantification (LOQ) fell in the range between 0.0691-1.04 μg/ml and 0.23–3.43 μg/ml, respectively. The mean recovery of each herbal medicine ranged from 96.60 to 102.11%. Conclusion: The method was validated for repeatability, precision, stability, accuracy, and selectivity. The validated method was successfully applied to simultaneous analysis of these active components in ‘Ge‑Gen‑Qin‑Lian’ decoction.
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An, R., You, L., Zhang, Y., Wang, X., & Ma, Y. (2014). A rapid UPLC method for simultaneous determination of eleven components in ‘Ge‑Gen‑Qin‑Lian’ decoction. Pharmacognosy Magazine, 10(40), 464–469. https://doi.org/10.4103/0973-1296.141821