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Article

Hplc‑ESI‑MS/MS analysis of the water‑soluble extract from Ziziphi spinosae semen and its ameliorating effect of learning and memory performance in mice

Yanqing Zhang2,3★, Longdong Qiao2, Mengyuan Song2, Lijuan Wang2,3, Junbo Xie2,3, Hua Feng2★ Corresponding author

  1. 1Department of Pharmaceutical Egineering, College of Biotechnology and Food Science, Tianjin University of Commerce, China.
  2. 2Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, People’s Republic of, China.

CORRESPONDENCE

Yanqing Zhang

Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, People’s Republic of, China.

Received: 13-10-2013; Revised: 06-12-2013.

Volume 10, Issue 40 · pp. 509–516 · PUBLISHED 26 September 2014 · DOI: 10.4103/0973-1296.141777

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ABSTRACT

Background: Ziziphi Spinosae Semen (ZSS), the seed of Ziziphus jujuba var. spinosa (Bunge) Hu ex H. F. Chow., is a traditional herb for insomnia and anxiety in eastern Asia. However, few researches have been concerned with its effect on ameliorating memory and learning performance. Objective: To investigate the constituents of ZSS water soluble extract and its ameliorating learning and memory in mice. Materials and Methods: A new high performance liquid chromatography coupled with tandem mass spectrometry (HPLC‑ESI‑MS/MS) method was developed and validated to determine the main constituents in the extract. The effect of ZSS water soluble extract on memory and learning performance was investigated in mice by Y‑maze and passive avoidance test. Results: The extract could significantly decrease the number of errors (NOE), and increase the transfer latency time (TLT) and electrical stimuli time (EST). In addition, spinosin, jujuboside A (JuA) and jujuboside B (JuB) were simultaneously identified and quantified in the extract. Their contents in the extract were as followed: Spinosin (223.51mg/g), JuA (63.76mg/g) and JuB (26.29mg/g). Conclusion: The extract played a promising role in ameliorating memory in mice with alcohol induced memory retrieval disorders, and might help to improve learning capacity to some extent. Spinosin, JuA and JuB were the predominant constituents, which might be mainly responsible for the definite activity.

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    Zhang, Y., Qiao, L., Song, M., Wang, L., Xie, J., & Feng, H. (2014). Hplc‑ESI‑MS/MS analysis of the water‑soluble extract from Ziziphi spinosae semen and its ameliorating effect of learning and memory performance in mice. Pharmacognosy Magazine, 10(40), 509–516. https://doi.org/10.4103/0973-1296.141777