Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages
Masoumeh Tangestani Fard1, Palanisamy Arulselvan2, Govindarajan Karthivashan2, Siti Khadijah Adam1, Sharida Fakurazi1,2★★ Corresponding author
- 1Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
- 2Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
CORRESPONDENCE
Sharida Fakurazi
Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Volume 11, Issue 44S3 · pp. 556–63 · PUBLISHED 2015 · DOI: 10.4103/0973-1296.172961
ABSTRACT
Introduction: Inflammation is a well‑known physiological response to protect the body against infection and restore tissue injury. Nevertheless, the chronic inflammation can trigger various inflammatory associated diseases/disorder. Moringa oleifera is a widely grown plant in most tropical countries and it has been recognized traditionally for several medicinal benefits. Objectives: The objective of this study was to investigate the anti‑inflammatory properties of M. oleifera extract on lipopolysaccharide (LPS) ‑ stimulated macrophages. Materials and Methods: The anti‑inflammatory effect of M. oleifera hydroethanolic bioactive leaves extracts was evaluated by assessing the inhibition of nitric oxide (NO) production during Griess reaction and the expression of pro‑inflammatory mediators in macrophages. Results: Interestingly, we found that M. oleifera hydroethanolic bioactive leaves extract significantly inhibited the secretion of NO production and other inflammatory markers such as prostaglandin E2, tumor necrosis factor alpha, interleukin (IL)‑6, and IL‑1β. Meanwhile, the bioactive extract has induced the production of IL‑10 in a dose‑dependent manner. In addition, M. oleifera hydroethanolic bioactive leaves extract effectively suppressed the protein expression of inflammatory markers inducible NO synthase, cyclooxygenase‑2, and nuclear factor kappa‑light‑chain‑enhancer of activated B‑cells p65 in LPS‑induced RAW264.7 macrophages in a dose‑dependent manner. Conclusion: These findings support the traditional use of M. oleifera plant as an effective treatment for inflammation associated diseases/disorders.
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Fard, M. T., Arulselvan, P., Karthivashan, G., Adam, S. K., & Fakurazi, S. (2015). Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages. Pharmacognosy Magazine, 11(44S3), 556–63. https://doi.org/10.4103/0973-1296.172961