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Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules

Karuppiah Shanmugasundarapandian Jayachandran1, A. Hannah Rachel Vasanthi2★, Narasimman Gurusamy3★ Corresponding author

  1. 1Department of Biotechnology, Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu, India.
  2. 2Department of Biotechnology, Pondicherry University, Puducherry, India.
  3. 3Department of Pharmacology, College of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia.

CORRESPONDENCE

A. Hannah Rachel Vasanthi

Department of Biotechnology, Pondicherry University, Puducherry, India.

hrvasanthi@gmail.com

Volume 12, Issue 45S · pp. S14–S20 · PUBLISHED 2016 · DOI: 10.4103/0973-1296.176114

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ABSTRACT

Background: Diosgenin, a steroidal saponin from plants, exhibits many biological potentials. Herein, the cardioprotective role of diosgenin is studied. Materials and Methods: The effect of diosgenin, isolated from Dioscorea bulbifera, was studied on hypoxia-reoxygenation (HR) in H9c2 cardiomyoblast cells. The amount of diosgenin in the plant extract was analyzed by high-performance thin layer chromatography using a solvent system comprising of chloroform:methanol:acetic acid:formic acid (13:4.5:1.5:1). Cardioprotection was checked by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Further, the release of lactate dehydrogenase, an enzyme released during cell death was checked. The proteins responsible for cell death (Bax) and cell survival (Bcl-2, hemeoxygenase-1 and Akt) were analyzed using Western blot to check the cardioprotective role of diosgenin. Conclusion: Supplementation of diosgenin mitigates HR injury, thereby exhibiting cardioprotective potential.

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    Jayachandran, K. S., Vasanthi, A. H. R., & Gurusamy, N. (2016). Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules. Pharmacognosy Magazine, 12(45S), S14–S20. https://doi.org/10.4103/0973-1296.176114