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Expression of Cytochrome P450s in the Liver of Rats Administered with Socheongryong‑tang, a Traditional Herbal Formula

Seong Eun Jin1, Hyekyung Ha2, Chang‑Seob Seo2, Hyeun‑Kyoo Shin2, Soo‑Jin Jeong2★★ Corresponding author

  1. 1K‑herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305‑811, Republic of Korea.
  2. 2KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 305‑811, Republic of Korea.

CORRESPONDENCE

Soo‑Jin Jeong

KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 305‑811, Republic of Korea.

sjijeong@kiom.re.kr

Received: 02-04-2015; Revised: 20-05-2015; Accepted: 20-05-2016.

Volume 12, Issue 47 · pp. 211–218 · PUBLISHED 14 July 2016 · DOI: 10.4103/0973-1296.186340

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ABSTRACT

Objective: The purpose of this study was to investigate the potential influences of Socheongryong‑tang (SCRT) on the messenger ribonucleic acid (mRNA) and protein expression of cytochrome P450 (CYP450) in vivo. Materials and Methods: SCRT was orally administered to either male or female Sprague‑Dawley rats once daily at doses of 0, 1000, 2000, or 5000 mg/kg/day for 13 weeks. The mRNA expression of CYP450s (CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) in liver tissues was measured by reverse transcription polymerase chain reaction. And then, the protein expression of CYP1A1 and CYP2B1/2 in liver tissues was analyzed by the Western blot. Results: We found no significant influence in the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1 after repeated administration of SCRT for 13 weeks. By contrast, the mRNA and protein expression of hepatic CYP1A1 was increased by repeated SCRT treatment in male rats, but not in female rats. The mRNA and protein expression of hepatic CYP2B1/2 in both genders was increased by administration of SCRT. Conclusion: A caution is needed when SCRT is co‑administered with substrates of CYP2B1/2 for clinical usage. In case of male, an attention is also required when SCRT and drugs metabolized by CYP1A1 are taken together. Our findings provide information regarding the safety and effectiveness of SCRT when combined with conventional drugs.

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    Jin, S. E., Ha, H., Seo, C., Shin, H., & Jeong, S. (2016). Expression of Cytochrome P450s in the Liver of Rats Administered with Socheongryong‑tang, a Traditional Herbal Formula. Pharmacognosy Magazine, 12(47), 211–218. https://doi.org/10.4103/0973-1296.186340