High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn
Lu Zhao1, E Wen1, Halmuart Upur2, Shuge Tian3★★ Corresponding author
- 1College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, Xinjiang, China.
- 2College of TCM, Xinjiang Medical University, Urumqi, Xinjiang, China.
- 3Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China.
CORRESPONDENCE
Shuge Tian
Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China.
Received: 08-10-2015; Revised: 03-11-2015; Accepted: 03-11-2015.
Volume 13, Issue 49 · pp. 136–40 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197656
ABSTRACT
Context: Sea buckthorn (Hippophae rhamnoides L.) as a traditional Chinese medicinal plant has various uses in Xinjiang. Objective: A reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases. Settings and design: Compounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm. Materials and Methods: The fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province. Statistical performances: The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes (R2 > 0.9997). Results: The proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang. Conclusions: The proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn.
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Zhao, L., Wen, E., Upur, H., & Tian, S. (2017). High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn. Pharmacognosy Magazine, 13(49), 136–40. https://doi.org/10.4103/0973-1296.197656