Comparative Pharmacokinetic Profiles of Three Protoberberine-type Alkaloids from Raw and Bile-processed Rhizoma coptidis in Heat Syndrome Rats
Yuan Zi-min1, Chen Yue1, Gao Hui1, Lv Jia1, Chen Gui-rong1, Jing Wang1★★ Corresponding author
- 1College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
CORRESPONDENCE
Jing Wang
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
Received: 01-03-2016; Revised: 02-04-2016; Accepted: 02-04-2016.
Volume 13, Issue 49 · pp. 51–7 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197632
ABSTRACT
Background: The Bile-processed Rhizoma coptidis (BRC), which has a colder drug property than Rhizoma coptidis (RC), is widely used for the treatment of heat syndrome. We compared the pharmacokinetics of the protoberberine-type alkaloids in BRC and RC in rats with heat syndrome to elucidate the bile-processing mechanism. Material and Methods: We established a rapid and sensitive method for simultaneously determining three alkaloids: berberine, palmatine, and jatrorrhizine, in rat plasma based on ultra-performance liquid chromatography/tandem mass spectrometry. The separation was carried out on a Waters ACQUITY BEA C18 column. The mobile phase consisted of acetonitrile (containing 0.1% formic acid) and water (containing 0.1% formic acid and 10 mmol/L ammonium acetate) and carbamazepine was used as an internal standard. The detection was carried out in a multiple reaction monitoring mode (MRM) using electrospray ionization in the positive ion mode. Results: Pharmacokinetic profiles indicated that the Cmax of berberine and palmatine increased two times and the Tmax of the three alkaloids decreased three times after bile processing. AUC0→∞ and AUC0→t of the alkaloids were similar between RC and BRC. Conclusion: The results suggest that bile processing could increase the absorption rate of alkaloids. This study broadens our understanding of Chinese herbal medicine processing.
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Zi-min, Y., Yue, C., Hui, G., Jia, L., Gui-rong, C., & Wang, J. (2017). Comparative Pharmacokinetic Profiles of Three Protoberberine-type Alkaloids from Raw and Bile-processed Rhizoma coptidis in Heat Syndrome Rats. Pharmacognosy Magazine, 13(49), 51–7. https://doi.org/10.4103/0973-1296.197632