In vitro Anti-Proliferative Effect of Tephrosia purpurea on Human Hepatocellular Carcinoma Cells
Ramamoorthy Padmapriya1, Loganathan Gayathri2, Larance Ronsard3, Mohammad A. Akbarsha3, Ramasamy Raveendran3★★ Corresponding author
- 1Department of Pharmacology, JIPMER, Puducherry, India.
- 2Mahatma Gandhi-Doerenkamp Center, Bharathidasan University, Tiruchirappalli, India.
- 3Division of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA.
CORRESPONDENCE
Ramasamy Raveendran
Division of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA.
Received: 19-05-2016; Revised: 07-11-2016; Accepted: 07-11-2016.
Volume 13, Issue 49S · pp. 16–21 · PUBLISHED 7 April 2017 · DOI: 10.4103/0973-1296.203981
ABSTRACT
Background: Tephrosia purpurea is an Indian herb used in traditional medicine to treat various diseases such as jaundice, asthma, liver and urinary disorders. However, the anti-cancer potential of T. purpurea on hepatocellular carcinoma (HCC) is poorly understood. Therefore, this study aims to investigate the anti-cancer activity of T. purpurea in HepG2 hepatocellular carcinoma cells. Methods: The leaves and root of T. purpurea were extracted with methanol using soxhlet apparatus. The cytotoxicity of the T. purpurea extracts in HepG2 cells was evaluated using MTT assay whereas the mode of cell death was examined by AOEB, Hoechst and JC1 staining under a fluorescence microscope. T. purpurea extracts-induced caspase-3 expression was investigated using colorimetric assay. Results: The leaves and root extracts inhibited HepG2 cell growth at the IC50 of 102.33 ± 10.26 µg/mL and 276.67 ± 20.43 µg/mL respectively at 24 h. Chromatin condensation, nuclear fragmentation, apoptotic bodies formation and mitochondrial membrane depolarization were observed in HepG2 cells treated with both extracts. The caspase-3 expression was significantly (p < 0.05) increased in extracts treated cells when compared to control. Conclusion: The leaves and root extracts of T. purpurea induce apoptosis mediated cell death in HepG2 cells.
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Padmapriya, R., Gayathri, L., Ronsard, L., Akbarsha, M. A., & Raveendran, R. (2017). In vitro Anti-Proliferative Effect of Tephrosia purpurea on Human Hepatocellular Carcinoma Cells. Pharmacognosy Magazine, 13(49S), 16–21. https://doi.org/10.4103/0973-1296.203981