Simultaneous Determination of Multiple Ginsenosides in Panax ginseng Herbal Medicines with One Single Reference Standard
Chunwei Wu2, Qingxiao Guan2, Shumei Wang2,3,4★, Yueying Rong2★ Corresponding author
- 1Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, China.
- 2Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine, China.
- 3Engineering and Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangzhou 510006, P.R, China.
CORRESPONDENCE
Shumei Wang
Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine, China.
Received: 05-06-2016; Revised: 11-07-2016.
Volume 13, Issue 49S · pp. 84–89 · PUBLISHED 7 April 2017 · DOI: 10.4103/pm.pm_274_16
ABSTRACT
Background: Root of Panax ginseng C. A. Mey (Renseng in Chinese) is a famous Traditional Chinese Medicine. Ginsenosides are the major bioactive components. However, the shortage and high cost of some ginsenoside reference standards make it is difficult for quality control of P. ginseng. Objective: A method, single standard for determination of multicomponents (SSDMC), was developed for the simultaneous determination of nine ginsenosides in P. ginseng (ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, Rd). Materials and Methods: The analytes were separated on Inertsil ODS‑3 C18 (250 mm × 4.6 mm, 5 μm) with gradient elution of acetonitrile and water. The flow rate was 1 mL/ min and detection wavelength was set at 203 nm. The feasibility and accuracy of SSDMC were checked by the external standard method, and various high‑performance liquid chromatographic (HPLC) instruments and chromatographic conditions were investigated to verify its applicability. Using ginsenoside Rg1 as the internal reference substance, the contents of other eight ginsenosides were calculated according to conversion factors (F) by HPLC. Results: The method was validated with linearity (r2 ≥ 0.9990), precision (relative standard deviation [RSD] ≤2.9%), accuracy (97.5%–100.8%, RSD ≤ 1.6%), repeatability, and stability. There was no significant difference between the SSDMC method and the external standard method. Conclusion: New SSDMC method could be considered as an ideal mean to analyze the components for which reference standards are not readily available.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Wu, C., Guan, Q., Wang, S., & Rong, Y. (2017). Simultaneous Determination of Multiple Ginsenosides in Panax ginseng Herbal Medicines with One Single Reference Standard. Pharmacognosy Magazine, 13(49S), 84–89. https://doi.org/10.4103/pm.pm_274_16