Anti-inflammatory Effects of KOTMIN13: A Mixed Herbal Medicine in LPS-stimulated RAW 264.7 Cells and Mouse Edema Models
Eujin Lee1, Sun-Gun Kim1, Na-Young Park1, Hyo-Hyun Park1, Kyu-Tae Jeong1, Jongkeun Choi2, In-Hae Lee2, Hwadong Lee1, Eunkyung Lee1★★ Corresponding author
- 1Research and Development Division, National Development Institute of Korean Medicine, Gyeongsan.
- 2Department of Cosmetic Science, Chungwoon University, Chungnam, Republic of Korea.
CORRESPONDENCE
Eunkyung Lee
Research and Development Division, National Development Institute of Korean Medicine, Gyeongsan.
Received: 11-01-2016; Revised: 29-02-2016; Accepted: 29-02-2016.
Volume 13, Issue 50 · pp. 216–21 · PUBLISHED 18 April 2017 · DOI: 10.4103/0973-1296.204548
ABSTRACT
Background: A Korean herbal medicine, KOTMIN13, composed of Inula japonica Thunberg, Trichosanthes kirilowii Maximowicz var. japonica kitamura, Peucedanum praeruptorum Dunn, and Allium macrostemon Bge, has been used for anti-allergic and anti-asthmatic treatment in oriental clinics, but its activity has not been investigated. Materials and Methods: To evaluate the anti-inflammatory activity of KOTMIN13 for in vitro study, LPS-stimulated RAW 264.7 cells were used to induce the production and expression of inflammatory mediators and its mechanisms. 12-O-Tetradecanoylphorobol-13 aceate (TPA)-induced ear edema and carrageenan-induced paw edema models were also used to evaluate the effect of KOTMIN13 on acute inflammation in vivo. Results: KOTMIN13 reduced the release of inflammatory mediators [nitric oxide, prostaglandin E2, interleukin (IL)-1β, and IL-6] and the protein expression of inducible nitric oxide synthase and cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Mechanism studies showed the attenuation of LPS-induced NF-κB activation by KOTMIN13 via IκBα degradation abrogation and a subsequent decrease in nuclear p65 levels. Activation of mitogen-activated protein kinases (ERK, JNK, and p38) was also suppressed. Furthermore, KOTMIN13 ameliorated the development of TPA-induced ear edema and carrageenan-induced paw edema in acute inflammatory edema mouse models. Conclusion: Our study demonstrates that KOTMIN13 inhibits inflammatory mediators through the inhibitions of NF-κB and MAPK activities in LPS-induced RAW 264.7 cells, as well as acute inflammation in edema models, indicating that KOTMIN13 is an effective suppressor for anti-inflammatory activities.
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Lee, E., Kim, S., Park, N., Park, H., Jeong, K., Choi, J., Lee, I., Lee, H., & Lee, E. (2017). Anti-inflammatory Effects of KOTMIN13: A Mixed Herbal Medicine in LPS-stimulated RAW 264.7 Cells and Mouse Edema Models. Pharmacognosy Magazine, 13(50), 216–21. https://doi.org/10.4103/0973-1296.204548