Isolation of Abscisic Acid from Korean Acacia Honey with Anti-Helicobacter pylori Activity
SeGun Kim1, InPyo Hong1, SoonOk Woo1, HyeRi Jang1, SokCheon Pak2, SangMi Han1★★ Corresponding author
- 1Department of Agricultural Biology, National Institute of Agricultural Science, Wanju, Korea.
- 2School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW, Australia.
CORRESPONDENCE
SangMi Han
Department of Agricultural Biology, National Institute of Agricultural Science, Wanju, Korea.
Received: 08-05-2016; Revised: 07-11-2016; Accepted: 07-11-2016.
Volume 13, Issue 50S · pp. S170–3 · PUBLISHED 7 November 2017 · DOI: 10.4103/0973-1296.210166
ABSTRACT
Background: Helicobacter pylori (H. pylori) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide. Objective: This study is aimed at evaluating the anti-H. pylori activity of Korean acacia honey and isolating the related active components using organic solvents. Material and Methods: The crude acacia honey was extracted with n-hexane, dichloromethane, ethyl acetate (EtOAc), and n-butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti-H. pylori activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against H. pylori was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay. Results: Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36–72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against H. pylori. Conclusion: Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for H. pylori-induced infections.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Kim, S., Hong, I., Woo, S., Jang, H., Pak, S., & Han, S. (2017). Isolation of Abscisic Acid from Korean Acacia Honey with Anti-Helicobacter pylori Activity. Pharmacognosy Magazine, 13(50S), S170–3. https://doi.org/10.4103/0973-1296.210166