Biochemical Characterization of Fungus Isolated during In vitro Propagation of Bambusa balcooa
Bhawna Tyagi1★, Salil Tewari2, Ashutosh Dubey1★ Corresponding author
- 1Department of Biochemistry, College of Basic Sciences and Humanities, India.
- 2Agroforestry Research Centre, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
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Bhawna Tyagi
Department of Biochemistry, College of Basic Sciences and Humanities, India.
Received: 20-01-2017; Revised: 03-03-2017.
Volume 13, Issue 52S · pp. S775–S779 · PUBLISHED 31 January 2018 · DOI: 10.4103/pm.pm_20_17
ABSTRACT
Background: Bambusa balcooa (Poaceae: Bambusoideae) is a multipurpose bamboo species, which is native of the Indian subcontinent. B. balcooa is regarded as one of the best species for scaffolding and building purposes because of its strong culm. Other uses include paper pulp, handicrafts, and products of the wood chip industry. Due to these various uses in industries, this species has been identified as one of the priority bamboos by the National Bamboo Mission. Objective: This study is designed to analyze the identification of fungus and develop the strategy to eliminate the contamination during in vitro establishment of B. balcooa through nodal part. Fungus contamination is a problem which is encountered during in vitro establishment of B. balcooa cultures. Materials and Methods: In the present study, fungus contamination from in vitro cultured plant has been isolated and subjected to partial sequence analysis of the 18S rRNA gene to identify the fungus strain. Experiments were designed to develop a strategy for removal of the fungus contamination with the help of antifungal compounds and commercial antimicrobial supplement supplied by HiMedia. Results: Fusarium equiseti was identified as endophytic fungus. It was observed that antimicrobial supplement at concentration of 500 μl/l was more effective concentration to remove fungus contamination and not showed any detrimental effect on growth parameters of shoot. Conclusion: This experiment would help in identification and to get rid of fungal contamination and improve the in vitro establishment of B. balcooa cultures for large‑scale propagation.
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Tyagi, B., Tewari, S., & Dubey, A. (2018). Biochemical Characterization of Fungus Isolated during In vitro Propagation of Bambusa balcooa. Pharmacognosy Magazine, 13(52S), S775–S779. https://doi.org/10.4103/pm.pm_20_17