Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits
Palanigounder Ganeshan Ajay Krishna1, Thasma Raman Sivakumar1, Chao Jin1, Shao-Hui Li1, Yu-Jie Weng1, Juan Yin1, Jun-Qiang Jia1,2, Chu-Yan Wang3, Zhong-Zheng Gui1,2★★ Corresponding author
- 1Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
- 2Lab of Hi-Tech Processing for Sericultural Resources, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
- 3Department of Biological and Environmental Engineering, Hefei University, Hefei, Anhui 230601, China.
CORRESPONDENCE
Zhong-Zheng Gui
Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Received: 04-11-2016; Revised: 23-11-2016; Accepted: 23-11-2016.
Volume 14, Issue 53 · pp. 103–9 · PUBLISHED 20 February 2018 · DOI: 10.4103/pm.pm_491_16
ABSTRACT
Background: Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti‑inflammatory, and chemoprotective properties. Objectives: In this study, purification of the polyphenol‑rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protective in vivo and in vitro. Materials and Methods: Antioxidant activities in vitro was measured by quantifying its 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) scavenging activity, reducing power and Fe2+‑chelating ability. MPE was purified by high‑pressure liquid chromatography (HPLC) and analyzed individual polyphenols using liquid chromatography–mass spectrometry (LC‑MS)/MS. Results: The total polyphenol content was 147.69 ± 0.02 mg gallic acid equivalents (GAE)/g dried weight (DW) in the extract and 403.55 ± 0.02 mg GAE/g DW in the purified extract. Further identification by HPLC‑ultraviolet‑visible and LC‑MS/MS analysis indicated in MPE, an anthocyanin compound, cyanidin‑3‑O‑glucoside. With regard to in vitro assays, MPE possessed antioxidant effect, especially in Fe2+ chelating ability with an IC50 value of 1.016 mg/mL. The protective effects on mouse red blood cell hemolysis and lipid peroxidation ex vivo were dose and time dependent. Conclusion: It indicates that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects.
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Krishna, P. G. A., Sivakumar, T. R., Jin, C., Li, S., Weng, Y., Yin, J., Jia, J., Wang, C., & Gui, Z. (2018). Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits. Pharmacognosy Magazine, 14(53), 103–9. https://doi.org/10.4103/pm.pm_491_16