Anti‑Eczematic and Molecular Modeling of Anthraquinones Isolated from the Seeds of Asphodelus microcarpus Salzm. Viv. Growing in Egypt
Abd El‑Salam I. Mohammed2, Arafa Musa2,3★, Marwa S. Abu‑Bakr4, Hatem S. Abbass2★ Corresponding author
- 1Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al‑Azhar University.
- 2Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka, Aljouf, Saudi Arabia.
- 3Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al‑Azhar University, Cairo, Egypt.
CORRESPONDENCE
Arafa Musa
Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka, Aljouf, Saudi Arabia.
Received: 12-02-2019; Revised: 26-03-2019.
Volume 15, Issue 65 · pp. 586–591 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_67_19
ABSTRACT
Objective: The objective of the study is to investigate the anti‑eczematic activity, acute toxicity, and molecular modeling of A. microcarpus seeds. Materials and Methods: Nuclear magnetic resonance, ultraviolet, and mass spectroscopy were applied for characterization of isolated metabolites; induction of eczema was conducted by 2% and 0.2% w/v dinitrochlorobenzene in acetone; eczema was treated with topical application of the different seed extracts in the form of ointments (1% w/w); Swiss albino mice (25–30 g) were used for the determination of LD50 and anti‑eczematic effect. Docking studies were performed by Molecular Operating Environment software. Results: A. microcarpus seed extract exhibited promising ant‑eczematic activity, six anthraquinones were isolated from chloroform portion and characterized as 10,7`‑bichrysophanol (1), asphodelin (2), chrysophanol‑8‑O‑methyl ether (3), chrysophanol (4), physcion (5), and emodin (6). Compounds 1, 3, and 5 exerted significant anti‑eczematic effect. Conclusion: Six known anthraquinone derivatives were isolated and characterized for the first time from the seeds of A. microcarpus. Chloroform fraction (1% w/w) showed significant anti‑eczematic effect compared to standard mometasone furoate (0.1 w/w). The docking study proved the anti‑eczematic activity of anthraquinone content by their affinity to the target human histamine H1 receptor.
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Mohammed, A. E. I., Musa, A., Abu‑Bakr, M. S., & Abbass, H. S. (2019). Anti‑Eczematic and Molecular Modeling of Anthraquinones Isolated from the Seeds of Asphodelus microcarpus Salzm. Viv. Growing in Egypt. Pharmacognosy Magazine, 15(65), 586–591. https://doi.org/10.4103/pm.pm_67_19