Antioxidant Activity and Enhanced Cytotoxicity of Aqueous Mucuna pruriens L. Leaf Extract by Doxorubicin on Different Human Cancer Cell Lines
Vesna Tumbas‑Šaponjac1, Oke‑Oghene Philomena Akpoveso2★, Oluwatosin Imoleayo Oyeniran3, Jelena Desančić4, Dragana Četojević‑Simin4,5★ Corresponding author
- 1Department for Applied and Engineering Chemistry, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia, 1 Department of Pharmacology, School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, England, UK.
- 2Department of Physiology, College of Health Sciences, Nile University of Nigeria, Abuja, 3 Faculty of Medicine, University of Novi Sad, Novi Sad, 4 Department for Science, Research and Education, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia, Nigeria.
CORRESPONDENCE
Oke‑Oghene Philomena Akpoveso
Department of Physiology, College of Health Sciences, Nile University of Nigeria, Abuja, 3 Faculty of Medicine, University of Novi Sad, Novi Sad, 4 Department for Science, Research and Education, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia, Nigeria.
Received: 26-09-2019; Revised: 13-11-2019.
Volume 16, Issue 68S · pp. S224–S228 · PUBLISHED 31 March 2020 · DOI: 10.4103/pm.pm_413_19
ABSTRACT
Objective: The aim of this study was to evaluate the antioxidant and cytotoxic effect of an aqueous leaf extract of MP in different human cancer cell lines. This study also evaluated the enhanced cytotoxic effect of an aqueous leaf extract of MP with Doxorubicin (Dox) in the selected human cancer cell lines. Materials and Methods: In this study, the breast cancer cell lines (MCF‑7 and MDA‑MB‑231), cervix carcinoma cell line (HeLa), and colon cancer cell line (HT‑29) were used. As a control, the non-cancer lung cell line (MRC‑5) was used. Cytotoxicity was assessed using the sulforhodamine B method. Antioxidant activity was measured with 2,2’‑Azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid) diammonium salt, 2,2‑diphenyl‑1‑picrylhydrazyl, and ferric reducing antioxidant power assay. Results: Aqueous MP leaf exhibited cytotoxicity in all the cell lines. The highest cytotoxic activity of the test extract was observed in HeLa cells at half‑maximal inhibitory concentration (IC50) = 92.8 μg/ml. Furthermore, the IC50 value of the test extract when combined with Dox was significantly reduced. Specifically, in HeLa cells, the IC50 was reduced by approximately 40 fold. Conclusion: This study demonstrates that aqueous MP leaf extracts could be useful as a source of antioxidants and compounds for cancer therapy. Further research is required to evaluate the chemical constituents of the leaf extracts and potential benefits for cancer therapy.
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Tumbas‑Šaponjac, V., Akpoveso, O. P., Oyeniran, O. I., Desančić, J., & Četojević‑Simin, D. (2020). Antioxidant Activity and Enhanced Cytotoxicity of Aqueous Mucuna pruriens L. Leaf Extract by Doxorubicin on Different Human Cancer Cell Lines. Pharmacognosy Magazine, 16(68S), S224–S228. https://doi.org/10.4103/pm.pm_413_19