Rapid Identification of the Indigenous Medicinal Crop Cinnamomum osmophloeum from Various Adulterant Cinnamomum Species by DNA Polymorphism Analysis
Bo‑Cheng Yang1, Meng‑Shiunn Lee2★, Fang‑Chun Sun2, Heng‑Hsiang Chao1, Wen‑Te Chang1, Ming‑Kuem Lin1, Hsi‑Jien Chen1★ Corresponding author
- 1Meng‑Shiou Lee Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung, Taiwan.
- 2Department of Bioresources, Da‑Yeh University, Changhua, Taiwan.
- 3Department of Safety, Health and Environmental Engineering, Mingchi University of Technology, Taipei, Taiwan.
CORRESPONDENCE
Meng‑Shiunn Lee
Department of Bioresources, Da‑Yeh University, Changhua, Taiwan.
Received: 24-06-2019; Revised: 16-09-2019.
Volume 16, Issue 68S · pp. S64–S68 · PUBLISHED 31 March 2020 · DOI: 10.4103/pm.pm_267_19
ABSTRACT
Objective: The purpose of this study was to develop a DNA‑based molecular method for rapid authentication of the indigenous Co and prevention of its adulteration. Materials and Methods: The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from various Cinnamomum species were amplified by polymerase chain reaction (PCR), and these obtained sequences were used for sequence analysis. Based on the sequence variants among various Cinnamomum species, restriction fragment length polymorphism (RFLP) was used to differentiate these Cinnamomum plants. Results: Two restriction endonucleases, MylI and EcoRV, were specifically used to digest the PCR‑amplified ITS DNA from seven Cinnamomum species. The PCR‑RFLP results demonstrated that the different restriction patterns that were produced by these two restriction enzymes clearly distinguished Co from Cb and five other Cinnamomum species simultaneously. Conclusion: The PCR‑RFLP analysis developed in this study provides an alternative method for rapidly identifying Cinnamomum plants at the species level using DNA polymorphisms.
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Yang, B., Lee, M., Sun, F., Chao, H., Chang, W., Lin, M., & Chen, H. (2020). Rapid Identification of the Indigenous Medicinal Crop Cinnamomum osmophloeum from Various Adulterant Cinnamomum Species by DNA Polymorphism Analysis. Pharmacognosy Magazine, 16(68S), S64–S68. https://doi.org/10.4103/pm.pm_267_19