jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Article

Evaluation of Wound Healing Potential of Ascorbic Acid, Castor Oil, and Gum Tragacanth Formulation in Murine Excisional Wound Model

Ajay K. Sharma1★, Sandeep Kumar Shukla1★, Aman Kalonia1, Priyanka Shaw1, M. H. Yashavarddhan2, Kailash Manda1★ Corresponding author

  1. 1Innovative Research Group, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, India.
  2. 2Cardiorespiratory and High Altitude Physiology Division, Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organization, Delhi, India.

CORRESPONDENCE

Ajay K. Sharma

Innovative Research Group, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, India.

sandeepshukla@inmas.drdo.in

Received: 14-10-2019; Revised: 31-10-2019; Accepted: 26-12-2019.

Volume 16, Issue 69 · pp. 359–368 · PUBLISHED 15 June 2020 · DOI: 10.4103/pm.pm_440_19

View on PM original site ↗

ABSTRACT

Objectives: The objective of this study was to evaluate the potential of ascorbic acid, castor oil, and gum tragacanth formulation, as an effective wound healing composition. Materials and Methods: A formulation containing ascorbic acid (0.5%), tragacanth gum (6%), and castor oil (25%) is prepared and evaluated for its efficacy on full‑thickness mouse excisional wound model. Povidone‑iodine was used as a positive control for comparative efficacy. Morphological and histopathological studies were carried out for understanding its wound healing potential. Antibacterial action was tested using disk diffusion method. Safety assessment for oral toxicity and eye and skin irritation was investigated in mouse and rabbit models, respectively. Permeability studies of formulation with excised skin have been evaluated by diffusion chamber, and its absorption in skin and serum was measured with high‑performance liquid chromatography method. Results: Wound treated twice a day with formulation showed up to 24% recovery after 24 h and full skin restoration on the 9th day compared to the untreated wound. The morphological and histopathological examinations of wound indicated noteworthy improvement in recovery. Comparative efficacy showed 98% wound recovery in formulation‑treated groups compared to 89% with commercially available povidone‑iodine ointment after the 9th day. Permeability data clearly indicate enhanced skin uptake in time‑dependent manner. The formulation is found to be safe and effective as demonstrated by antibacterial nature, permeability, and supportive safety studies. Conclusion: This study elucidates this formulation possesses strong wound healing potential.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Sharma, A. K., Shukla, S. K., Kalonia, A., Shaw, P., Yashavarddhan, M. H., & Manda, K. (2020). Evaluation of Wound Healing Potential of Ascorbic Acid, Castor Oil, and Gum Tragacanth Formulation in Murine Excisional Wound Model. Pharmacognosy Magazine, 16(69), 359–368. https://doi.org/10.4103/pm.pm_440_19