Apigenin-induced Apoptosis is Reduced via Endoplasmic Reticulum Stress and ATF6/PERK Signaling in Human Gastric Cancer Cells
Cuizhu Zhuo1, Xiaolin Wang2, Yun Li1, Yongmei Tang1, Manman Wang1, Liying Hou1★★ Corresponding author
- 1School of Public Health, North China University of Science and Technology, Caofeidian, Tangshan, Hebei, China
- 2The Preventive Health Care Department, People’s Hospital in Liaocheng City, Liaocheng, China
CORRESPONDENCE
Liying Hou
School of Public Health, North China University of Science and Technology, No.21 Bohai Road, Caofeidian, Tangshan, Hebei, China.
Received: 18-01-2023; Accepted: 02-06-2023.
Volume 19, Issue 4 · pp. 898–905 · PUBLISHED 2023 · DOI: 10.1177/09731296231188792
ABSTRACT
Background: Apigenin effectively inhibits the growth of human gastric carcinoma SGC-7901 cells and induces apoptosis as well. This study investigated the effects of ATF6 and PERK signaling pathways in unfolded protein response (UPR) and endoplasmic reticulum stress (ERS) on the apoptosis induced by apigenin in human gastric carcinoma SGC-7901 cells. Materials and Methods: SGC-7901 cells were cultured with apigenin and tunicamycin for 48 h or with apigenin for 12–48 h. CCK-8 was used to determine cell viability. The mRNA expression level was detected by RT-qPCR. The expression of related proteins was explored by Western blot. The apoptosis rate of cells and cell-cycle arrest were evaluated by flow cytometer. Results: The results of CCK-8 confirmed that apigenin could induce apoptosis of SGC-7901 cells. In the apigenin-treated cells group, the protein and mRNA levels of GRP78 and GRP94 dose- and time-dependently increased. Additionally, apigeninactivated UPR components PERK and ATF6. However, apigenin exerted no influence on CHOP expression or JNK activation. Pretreatment with 4-PBA significantly increased the apigenin-triggered apoptosis in SCG-7901 cells (p < 0.05). Conclusion: The results revealed the protective effect of UPR performance on apigenin-triggered apoptosis in SGC-7901 cells.
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Zhuo, C., Wang, X., Li, Y., Tang, Y., Wang, M., & Hou, L. (2023). Apigenin-induced Apoptosis is Reduced via Endoplasmic Reticulum Stress and ATF6/PERK Signaling in Human Gastric Cancer Cells. Pharmacognosy Magazine, 19(4), 898–905. https://doi.org/10.1177/09731296231188792