Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the Activation of NLRP3 Inflammasome in BV2 Cells
Junli Wang1, Rui Wang2, Yaming Du2, Xinzi Xu2, Xin Liu1, Wei Shao1, Guohua Chen1,2★★ Corresponding author
- 1College of Clinical Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei, China
- 2Department of Neurology, Wuhan No. 1 Hospital, Wuhan, Hubei, China
CORRESPONDENCE
Guohua Chen
Department of Neurology, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.
Received: 13-09-2023; Accepted: 06-02-2024.
Volume 20, Issue 4 · pp. 1359–1366 · PUBLISHED 2024 · DOI: 10.1177/09731296241244575
ABSTRACT
Background: Huanglian Jiedu decoction (HLJDD) is a classical herbal formula for treating inflammatory diseases with Traditional Chinese Medicine (TCM). However, the impacts of HLJDD on neuroinflammation and the precise mechanisms involved remain poorly understood. Purpose: In the present study, HLJDD was found to inhibit lipopolysaccharide (LPS) induced neuroinflammatory responses in BV2 microglia via regulating NLRP3 inflammasome activation. Materials and Methods: Anti-inflammatory activity was evaluated with ELISA assay and the polarization changes were observed using immunofluorescent staining. The levels of NLRP3 inflammasome activation were determined using qPCR and Western blotting. Results: The administration of HLJDD successfully countered LPS-induced neuroinflammatory responses by attenuating microglial activation and fostering a phenotypic conversion from the pro-inflammatory M1 to the anti-inflammatory M2. Additionally, HLJDD treatment demonstrated inhibition of NLRP3 inflammasome activation. Conclusion: The findings of the present study may provide insights into how HLJDD could be a therapeutic drug for neuroinflammatory diseases associated with regulating NLRP3 inflammasome and microglial activation.
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Wang, J., Wang, R., Du, Y., Xu, X., Liu, X., Shao, W., & Chen, G. (2024). Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the Activation of NLRP3 Inflammasome in BV2 Cells. Pharmacognosy Magazine, 20(4), 1359–1366. https://doi.org/10.1177/09731296241244575