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Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze

Xinhui Tang2, Lifang Yan2,3, Jing Gao3★, Haiyan Ge3, Haidong Yang3, Na Lin3★ Corresponding author

  1. 1Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002, China.
  2. 2School of Pharmacy, Jiangsu University, Zhenjiang - 212013, People’s Republic of, China.

CORRESPONDENCE

Jing Gao

jinggao@ujs.edu.cn

Received: 31-01-2011; Revised: 13-03-2011.

Volume 7, Issue 27 · pp. 186–192 · PUBLISHED 25 August 2011 · DOI: 10.4103/0973-1296.84225

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ABSTRACT

Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) Materials and Methods: One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2‘-Azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro. Results: According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity. Conclusion: One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.

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    Tang, X., Yan, L., Gao, J., Ge, H., Yang, H., & Lin, N. (2011). Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze. Pharmacognosy Magazine, 7(27), 186–192. https://doi.org/10.4103/0973-1296.84225