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Article

Inorganic ions in the medium modify tropane alkaloids and ribofl avin output in Hyoscyamus niger root cultures

Katrin Pudersell2★, Tõnis Vardja3, Rael Vardja3, Vallo Matto2, Elmar Arak2,4, Ain Raal2,4★ Corresponding author

  1. 1Department of Pharmacy, University of Tartu, Tartu, Estonia.
  2. 2Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Harku, Estonia.
  3. 3Estonian Academical Society of Pharmacy, Tartu, Estonia.

CORRESPONDENCE

Katrin Pudersell

Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Harku, Estonia.

Received: 12-04-2011; Revised: 18-05-2011.

Volume 8, Issue 29 · pp. 73–77 · PUBLISHED 28 February 2012 · DOI: 10.4103/0973-1296.93330

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ABSTRACT

Context: Hyoscyamus niger L. (Solanaceae) roots are rich of tropane alkaloids, such as hyoscyamine and scopolamine are used as the source of raw material for the pharmaceutical industry. Aims: The aim of the present study was to investigate the effect of calcium, magnesium, and iron ions on the production of tropane alkaloids and excretion of ribofl avin in H. niger root cultures. Materials and Methods: The calcium, magnesium, or iron enriched/deprived Murashige and Skoog (MS) growth medium were used for culture of H. niger root tissues. The secondary metabolites were quantifi ed using high performance liquid chromatography with ultraviolet detector (HPLC-UV) and fl uorimetry techniques. Results: An increased calcium content in the medium unidirectionally reduced hyoscyamine, while increasing scopolamine production with only a moderate impact on ribofl avin excretion. Manipulations with magnesium and iron contents in the medium resulted in divergent changes in hyoscyamine, scopolamine, and ribofl avin concentrations. Conclusions: Our results show that increased calcium ion content in the Murashige and Skoog medium may be used for the intensifi cation of the scopolamine production in H. niger root cultures.

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    Pudersell, K., Vardja, T., Vardja, R., Matto, V., Arak, E., & Raal, A. (2012). Inorganic ions in the medium modify tropane alkaloids and ribofl avin output in Hyoscyamus niger root cultures. Pharmacognosy Magazine, 8(29), 73–77. https://doi.org/10.4103/0973-1296.93330