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Antimicrobial activity of Marcetia DC species (Melastomataceae) and analysis of its flavonoids by reverse phase-high performance liquid chromatography coupled-diode array detector

Tonny Cley Campos Leite1★, Amanda Reges de Sena2, Tânia Regina dos Santos Silva3, Andrea Karla Almeida dos Santos4, Ana Paula Trovatti Uetanabaro5, Alexsandro Branco1★ Corresponding author

  1. 1Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
  2. 2Federal Institute for Education, Science and Technology of Pernambuco - Campus Barreiras, Barreiras, Pernambuco, Brazil.
  3. 3Department of Biological Sciences, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
  4. 4Institute of Multidisciplinary Health - Campus Anísio Teixeira, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.
  5. 5Microbiology Laboratory of Agribusiness, Department of Biological Sciences, State University of Santa Cruz, Ilhéus, Bahia, Brazil.

CORRESPONDENCE

Tonny Cley Campos Leite

Laboratory of Phytochemistry, Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.

Received: 07-09-2011; Revised: 05-10-2011.

Volume 8, Issue 31 · pp. 209–214 · PUBLISHED 2 August 2012 · DOI: 10.4103/0973-1296.99286

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ABSTRACT

Background: Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil), and most are endemic to the highlands of the Chapada Diamantina (Bahia). Among the species, only M. taxifolia (A.St.-Hil.) DC. populates Brazil (state of Roraima to Paraná) and also Venezuela, Colombia, and Guyana. Objective: This work evaluated the antimicrobial activity of hexane, ethyl acetate, and methanol extracts of three species of Marcetia (Marcetia canescens Naud., M. macrophylla Wurdack, and M. taxifolia A.StHil) against several microorganism. In addition, the flavonoids were analyzed in extracts by HPLC-DAD. Materials and methods: The tests were made using Gram-positive (three strains of Staphylococcus aureus) and Gram-negative (two strains of Escherichia coli, a strain of Pseudomonas aeruginosa and another of Salmonella choleraesius) bacteria resistant and nonresistant to antibiotics and yeasts (two strains of Candida albicans and one of C. parapsilosis) by the disk diffusion method. Solid-phase extraction (SPE) was performed on the above extracts to isolate flavonoids, which were subsequently analyzed by high performance liquid chromatography coupled diode array detector (HPLC-DAD). Results: Results showed that extracts inhibited the Gram-positive bacteria and yeast. The hexane extracts possessed the lowest activity, while the ethyl acetate and methanolic extracts were more active. Conclusion: Marcetia taxifolia was more effective (active against 10 microorganisms studied), and only its methanol extract inhibited Gram-negative bacteria (P. aeruginosa and S. choleraesius). SPE and HPLC-DAD analysis showed that M. canescens and M. macrophylla contain glycosylated flavonoids, while the majority of extracts from M. taxifolia were aglycone flavonoids.

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    Leite, T. C. C., Sena, A. R. D., Silva, T. R. D. S., Santos, A. K. A. D., Uetanabaro, A. P. T., & Branco, A. (2012). Antimicrobial activity of Marcetia DC species (Melastomataceae) and analysis of its flavonoids by reverse phase-high performance liquid chromatography coupled-diode array detector. Pharmacognosy Magazine, 8(31), 209–214. https://doi.org/10.4103/0973-1296.99286