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Article

Nimodipine rescues N-methyl-N-nitrosourea-induced retinal degeneration in rats

Dan Wang2,4, Yan Li3, Zhuo Wang4, Gui-yuan Sun2★, Qiu-hua Zhang5★ Corresponding author

  1. 1Department of Histology and Embryology, College of Basic Medical Science, China Medical University, China.
  2. 2Department of Physiology, Experimental Teaching Center of Pharmacology, School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, China.
  3. 3Department of Pharmacology, HE’s University, China.
  4. 4Department of Basic Pharmacology, Liaoning University of Traditional Chinese Medicine, Liaoning Shenyang, P. R, China.

CORRESPONDENCE

Gui-yuan Sun

Department of Physiology, Experimental Teaching Center of Pharmacology, School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, China.

sunguiyuan@163.com

Received: 14-02-2012; Revised: 29-02-2012.

Volume 9, Issue 34 · pp. 149–154 · PUBLISHED 30 April 2013 · DOI: 10.4103/0973-1296.111276

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ABSTRACT

Background: That nimodipine (NMD) is potentially useful for ophthalmic treatment. However, the effect of NMD is unknown on retinal degenerative diseases. Objective: The purpose of the present study was to investigate the effect of NMD on N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) and elucidate its possible mechanisms. Materials and Methods: Morphological observation of NMD on MNU-induced RD was evaluated by light microscopy and electron microscopy. Nonenzymatic antioxidant glutathione (GSH) was measured by a colorimetric method. Transforming growth factor-beta (TGF-b) was measured by enzyme-linked immunosorbent assay (ELISA). Telomerase was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The significantly protective effect of NMD on MNU-induced RD was demonstrated morphologically. NMD increased the content of GSH and decreased the level of TGF-b in rat retina. RT-PCR analysis demonstrated that NMD treatment significantly decreased mRNA level of telomerase. Conclusion: These data suggest that NMD inhibit MNU-induced RD in rats. The expressions of TGF-b, telomerase and GSH contents might partially contribute to its protective effects on MNU-induced RD.

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    Wang, D., Li, Y., Wang, Z., Sun, G., & Zhang, Q. (2013). Nimodipine rescues N-methyl-N-nitrosourea-induced retinal degeneration in rats. Pharmacognosy Magazine, 9(34), 149–154. https://doi.org/10.4103/0973-1296.111276