GC-MS analysis of Phytocomponents in the Methanol Extract of Premna latifolia Roxb

ABSTRACT

Background:According to existing literature, the genus Premnahas a variety of biologically active secondary metabolites, but there are few findings on phytochemical screening of P. latifoliaRoxb. Methanolic leaves extract. Objectives: Phytochemical screening of methanolic leaves extract of P. latifolia was proposed for this study. Materials and Methods:UV-chamber, HPTLC instrument (CAMAG TLC Scanner), and GC-MS instrument (Perkin-Elmer GC- Clarus) were used to analyse methanolic leaves extract of P. latifolia. To compare the peaks of components on chromatograms, the NIST library was employed.Results:The current study used GC-MS to identify probable chemical components of P. latifolia. The GC-MS analysis and NIST library comparison revealed that the methanolic extract of P. latifolia contained mainly Squalene (13.57 percent), Ergosta-5, 7, 9 (11), 22-tetraen-3-ol, (3. beta, 22E)-(0.15 percent), Stigmasterol (3.73 percent), gamma-Sitosterol (10.13 percent), Lupeol (0.33), beta-Amyrin (2.27 percent), alpha-Amyrin (2.05 percent), gamma-Sitostenone (0.35 percent), Ursolic aldehyde (1.01 percent) and Betulin (0.72 percent). The biological actions of the majority of the identified components have been reported. While scientific evidence of gamma-sitostenone’s biological function is still not available. Conclusion: According to the findings, P. latifolia contains a variety of biologically active components, the majority of which are tri-terpenoids and phytosterols.

KEYWORDS

• GC-MS Analysis
• HPTLC
• Methanolic leaves extract
• Phytocomponents TLC
• Premna latifolia

Showing references and in-text citations exactly as published.

• DOI23Copy

RELATED ARTICLES

Matched on section and article type within this issue of PR.

• Article · VOLUME 14, ISSUE 1Preventive Effects of Ginger Extract and Nigella sativa Oil on Anxiety and Depression Behavior in Wistar Rats Exposed to Mercuric ChlorideSabri Benkermiche, Samir Djemli, Meriem Haloui, Mohamed Lamine Bena bed, Abdelkrim Tahraoui · pp. 1–4Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 1Preliminary Assessment of Glycemic Control and Body Fat Reduction Effects of Terminalia chebula Retz. Extract on Pre-diabetic SubjectsPrasoborn Rinthong, Sansanee Sombattera, Bunleu Sungthong · pp. 5–11Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 1Virtual Screening of Fargesin Analogs as Candidates as Inhibitors of Aedes aegypti Sterol Carrier ProteinSammya Yasmin EM De Lima, Anderson Roberto Nery Da Silva, Cíntia Emi Yanaguibashi Leal, Edilson B De Alencar Filho · pp. 12–18Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 1Analgesic, Anti-inflammatory, and Anti-pyretic Activities of Crinum pedunculatum R.Br. Bulb ExtractsPeace Doe, Cynthia Amaning Danquah, Kwasi Adomako Ohemeng, Ako Ernest Opare, Ahmed Sharif, Dorcas Akua-Abora, Asamoah Kwame Akuoko, Aaron Kpabitey, Emmanuel Quarshie, Oliver Opoku Asante, Elvis Kwadwo Amponsah, Mariyana Mutwaliku, Charlotte Nuro-Brefo, Micheal Ofori · pp. 24–29Original ArticleSame type: Article