Piper pedicellatum C. DC. Leaf Methanol Extract Inhibits Edema and Granuloma and Modulates Mediators and Cytokines in Rats
Dev Jyoti Kalita1,2★, Kangkan Deka1,3, Ngurzampuii Sailo1, Sanjoy Das1, Bibhuti Bhusan Kakoti1★ Corresponding author
- 1Department of Pharmaceutical Sciences, School of Science and Engineering, Dibrugarh University, Dibrugarh, Assam, INDIA.
- 2Department of Medicine, Assam Medical College and Hospital, Dibrugarh, Assam, INDIA.
- 3Department of Pharmacognosy, NETES Institute of Pharmaceutical Science, Guwahati. Assam, INDIA.
CORRESPONDENCE
Dev Jyoti Kalita
Department of Pharmaceutical Sciences, School of Science and Engineering, Dibrugarh University, Dibrugarh, Assam, INDIA.
Received: 02-03-2023; Revised: 23-05-2023; Accepted: 18-06-2023.
Volume 15, Issue 4 · pp. 679–686 · PUBLISHED · DOI: 10.5530/pres.15.4.072
ABSTRACT
Background:Piper pedicellatum is an ethnomedicinal plant used in various inflammatory conditions by many ethnic groups and exploration of its therapeutic potentials is imperative. Objective:This study was carried out to assess anti-inflammatory potential of the methanol extract of the plant leaves using in vivo experiments to validate the traditional uses. Materials and Methods: Carrageenan-induced paw edema and Cotton pellet-induced granuloma models were used to investigate into the anti-inflammatory potential of the plant extract in rats at 200 mg/kg and 400 mg/kg dose. Indomethacin (10 mg/kg) is used as reference standard. Serum levels of inflammatory markers and cytokines, hematological and serum hepatic enzyme indices were analyzed in granuloma-induced model. Results:The methanol extract suppressed Carrageenan-induced paw edema significantly at different time intervals. The highest percentage inhibition was observed at 3rd hr after Carrageenan injection, which was 42.67% and 49.33% for 200 mg/kg and 400 mg/kg dose of the extract respectively. In granuloma-induced model, the methanol extract at 200 mg/kg and 400 mg/kg dose inhibited exudate formation by 28.58% and 41.27%, respectively and inhibition of granuloma tissue was 32.45% and 43.71%, respectively along with significant decrement in serum C-reactive protein, Prostaglandin E2, Tumor necrosis factor-α, Interleukin-6 while increases in Interleukin-10 levels. Also, the results showed hematological and serum hepatic enzyme levels were rectified to near normal levels in treated rats. Conclusion:The outcome of the investigation reveals the potential of extract as possible anti-inflammatory agent, which can be develop into therapeutic agent or supplement and also necessitates further pharmacological studies on the plant.
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Kalita, D. J., Deka, K., Sailo, N., Das, S., & Kakoti, B. B. (). Piper pedicellatum C. DC. Leaf Methanol Extract Inhibits Edema and Granuloma and Modulates Mediators and Cytokines in Rats. Pharmacognosy Research, 15(4), 679–686. https://doi.org/10.5530/pres.15.4.072