Standardised herbal extract of chlorogenic acid from leaves of Etlingera elatior (Zingiberaceae)
Eric W. C. Chan1★, Yau Yan Lim2, Shiau Pin Tan2★ Corresponding author
- 1Faculty of Applied Sciences, UCSI University, Cheras, Kuala Lumpur
- 2School of Science, Monash University Sunway Campus, Petaling Jaya, Selangor, Malaysia
CORRESPONDENCE
Eric W. C. Chan
*Address for correspondence: Dr. Eric W.C. Chan, Faculty of Applied Sciences, UCSI University, 56000 Cheras, Kuala Lumpur, Malaysia.
Revised: 25-06-2011.
Volume 3, Issue 3 · pp. 178–184 · PUBLISHED January 2011 · DOI: 10.4103/0974-8490.85003
ABSTRACT
Background Chlorogenic acid (CGA) or 5-caffeoylquinic acid, was found to be the dominant phenolic compound in leaves of Etlingera elatior (Zingiberaceae). The CGA content of E. elatior leaves was significantly higher than flowers of Lonicera japonica (honeysuckle), the commercial source. In this study, a protocol to produce a standardised herbal CGA extract from leaves of E. elatior using column chromatography was developed. Materials and Methods Freeze-dried leaves of E. elatior were extracted with 30% ethanol, and sequentially fractionated using Diaion HP-20 and Sephadex LH-20. Results The CGA fractions, which yielded extracts of 10% and 40% w/w purity, possessed antioxidant, tyrosinase inhibition, and antibacterial properties. The entire fractionation process took only 6.5 hours, using gravity flow. From 50 g of leaves, the final yield of CGA extract was 0.2 g (0.4%). The CGA content of the standardised herbal extract from leaves of E. elatior (40%) is 1.6 times that of commercial extracts from honeysuckle flowers (25%). Conclusion With high CGA content, the standardised herbal extract has a great potential to be developed into functional food and other health products. Leaves of E. elatior, which currently have no economic value, could serve as an alternative source of CGA. Leaves are large, available in abundance, and harvesting is non-destructive to the plants.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Chan, E. W. C., Lim, Y. Y., & Tan, S. P. (2011). Standardised herbal extract of chlorogenic acid from leaves of Etlingera elatior (Zingiberaceae). Pharmacognosy Research, 3(3), 178–184. https://doi.org/10.4103/0974-8490.85003