jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacognosy ResearchNOW IN
Article

Presence of monoterpene synthase in four Labiatae species and Solid-Phase Microextraction- Gas chromatography-Mass Spectroscopy analysis of their aroma profiles

Soodabeh Saeidnia1,2★, Ahmad Reza Gohari1,2, Azita Haddadi2, Gholamreza Amin1,3, Marjan Nikan1, Abbass Hadjiakhoondi1,3★ Corresponding author

  1. 1Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
  2. 2Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Canada
  3. 3Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

CORRESPONDENCE

Soodabeh Saeidnia

*Address for correspondence: Dr. Soodabeh Saeidnia, Medicinal Plants Research Center, Tehran University of Medical Sciences, P. O. Box 14155-6451, Tehran, Iran.

saeidnia_s@sina.tums.ac.ir

Revised: 17-08-2013.

Volume 6, Issue 2 · pp. 138–142 · PUBLISHED April-June 2014 · DOI: 10.4103/0974‑8490.129033

View on PR original site ↗

ABSTRACT

Background The family Lamiaceae (Labiatae) has included some medicinal plants. some monoterpene synthases, including linalool and limonene synthases, have been cloned and functionally characterized from several plants of Labiatae family. Materials and Methods In this study, presence of linalool and limonene synthases, in four species of Labiatae family including Nepeta cataria, Lavandula angustifolia, Hyssopus officinalis and Salvia sclarea has been determined by molecular biological techniques together with the Head space SPME – GC-MS analysis of the aroma profile of these species. Results Indicated that none of the plant species produced distinguishable bands with primer pairs related to d-limonene synthase. Distinguishable bands around 1800 bp in cDNA samples of L. angustifolia, H. officinalis and S. sclarea were observed regarding to the presence of linalool synthase. Head space SPME-GC-MS analysis of the aroma profiles of the above-mentioned plants showed that linalool (31.0%), linalyl acetate (18.2%), were found as the major compounds of L. angustifolia, while geraniol (5.5%), nerol (34.0%) and a- citral (52.0%) were identified as the main compounds of the N. cataria. The major components of H. officinalis and S. sclarea oils were determined as cis-pinocamphone (57.3%), and linalool (19.0%), linalyl acetate (51.5%), respectively. Conclusion H. officinalis was rich of cyclic monoterpenes, L. angustifolia, N. cataria and S. sclarea showed considerable amount of linear monoterpenes. The aroma profile of the above-mentioned plants contained low concentration of sesquiterpenes except N. cataria, which indicated no sesquiterpene. The profiles of the main components of these plants are in agreement with molecular assays.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Saeidnia, S., Gohari, A. R., Haddadi, A., Amin, G., Nikan, M., & Hadjiakhoondi, A. (2014). Presence of monoterpene synthase in four Labiatae species and Solid-Phase Microextraction- Gas chromatography-Mass Spectroscopy analysis of their aroma profiles. Pharmacognosy Research, 6(2), 138–142. https://doi.org/10.4103/0974‑8490.129033