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Pharmacognosy ResearchNOW IN
Article

Effects of Five Bangladeshi Plant Extracts on In vitro Thrombolysis and Cytotoxicity

Raju Dash1, Talha Bin Emran1,2, Arkajyoti Paul1, Mohammad Kutub Uddin Siddique1, Mohammad Arfad Khan1, Md. Golamur Rahman1, Md. Shahid Sarwar4, Mir Muhammad Nasir Uddin3★★ Corresponding author

  1. 1Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4000
  2. 2Departments of Biochemistry and Molecular Biology
  3. 3Pharmacy, University of Chittagong, Chittagong-4331
  4. 4Department of Pharmacy, Southeast University, Banani, Dhaka-1213, Bangladesh, India

CORRESPONDENCE

Mir Muhammad Nasir Uddin

Department of Pharmacy, Faculty of Biological Science, University of Chittagong, Chittagong-4331, Bangladesh.

nasirmir@cu.ac.bd

Volume 8, Issue 3 · pp. 176–180 · PUBLISHED Jul-Sep 2016 · DOI: 10.4103/0974-8490.181403

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ABSTRACT

Background Piper Betle, Pterospermum acerifolium, Saraca indica, Argyreia speciosa, and Rhaphidophora glauca are medicinal plants commonly used as traditional medicine for the treatment of various diseases. To examine, whether organic extracts of these plants possess thrombolytic properties with minimal or no toxicity is our main aim of the study. Materials and Methods In vitro thrombolytic model was used to check the clot lysis effects using streptokinase (SK) as a positive control and water as a negative control. Cytotoxicity was screened by brine shrimp lethality bioassay using vincristine sulfate (VS) as positive control. Results Among herbal drugs, different organic extracts of P. betle, P. acerifolium, S. indica, A. speciosa, and R. glauca showed significant (P < 0.05 and P < 0.0001) clot lysis activity viz., 31.58% ±0.76%, 40.50% ±0.94%, 49.70% ±1.69%, 35.81% ±0.86%, and 43.80% ±0.91%, respectively, compared to reference drug SK (79.32% ±1.629%). In brine shrimp cytotoxic assay, mortality achieved by the extracts showed lethal concentration 50 (LC50) values 274.64 ± 3.46, 215.60 ± 4.59, 478.40 ± 6.98, 233.37 ± 2.56, and 209.32 ± 1.98 mg/ml, respectively, with reference to VS (LC50, 0.05 ± 0.34). Conclusion In this study, S. indica, R. glauca, and P. acerifolium possessed effective thrombolytic activity. Further studies can be undertaken to identify certain structure of the ingredients in the extracts and to elucidate the precise mechanism of action.

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    Dash, R., Emran, T. B., Paul, A., Siddique, M. K. U., Khan, M. A., Rahman, M. G., Sarwar, M. S., & Uddin, M. M. N. (2016). Effects of Five Bangladeshi Plant Extracts on In vitro Thrombolysis and Cytotoxicity. Pharmacognosy Research, 8(3), 176–180. https://doi.org/10.4103/0974-8490.181403