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Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy

Gaurav M. Doshi1,4★, Vivek V. Nalawade1, Aaditi S. Mukadam2, Pratip K. Chaskar1, Sandeep P. Zine1, Rakesh R. Somani3, Hemant D. Une5★ Corresponding author

  1. 1Departments of Pharmacology and Pharmaceutical Chemistry
  2. 2Departments of Quality Assurance
  3. 3Pharmaceutical Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
  4. 4Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
  5. 5Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, India

CORRESPONDENCE

Gaurav M. Doshi

Asst. Prof. Gaurav M. Doshi, Department of Pharmacology, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra, India.

gaurav.pharmacology@gmail.com

Volume 8, Issue 4 · pp. 281–286 · PUBLISHED Oct-Dec 2016 · DOI: 10.4103/0974-8490.186578

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ABSTRACT

Background Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.

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    Doshi, G. M., Nalawade, V. V., Mukadam, A. S., Chaskar, P. K., Zine, S. P., Somani, R. R., & Une, H. D. (2016). Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy. Pharmacognosy Research, 8(4), 281–286. https://doi.org/10.4103/0974-8490.186578